BMRB Entry 6934

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6934
MolProbity Validation Chart

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Title:
1H, 15N, and 13C Assigned Chemical shift for Wzb
Deposition date:
2006-01-09
Original release date:
2007-02-06
Authors:
Lescop, Ewen; Jin, Changwen
Citation:

Citation: Lescop, Ewen; Hu, Yunfei; Xu, Huimin; Hu, Wei; Chen, Juan; Xia, Bin; Jin, Changwen. "The solution structure of Escherichia coli Wzb reveals a novel substrate recognition mechanism of prokaryotic low molecular weight protein tyrosine phosphatases"  J. Biol. Chem. 281, 19570-19577 (2006).
PubMed: 16651264

Assembly members:

Assembly members:
Wzb, polymer, 167 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Wzb: MGSSHHHHHHSSGLVPRGSH MFNNILVVCVGNICRSPTAE RLLQRYHPELKVESAGLGAL VGKGADPTAISVAAEHQLSL EGHCARQISRRLCRNYDLIL TMEKRHIERLCEMAPEMRGK VMLFGHWDNECEIPDPYRKS RETFAAVYTLLERSARQWAQ ALNAEQV

Data sets:
Data typeCount
13C chemical shifts592
15N chemical shifts163
1H chemical shifts1051

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Wzb monomer1

Entities:

Entity 1, Wzb monomer 167 residues - Formula weight is not available

The sequence reported here contains the 147 amino acid residue of Wzb. An 20aa N-terminal His-Tag was present in the construct.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METPHEASNASNILELEUVALVALCYSVAL
4   GLYASNILECYSARGSERPROTHRALAGLU
5   ARGLEULEUGLNARGTYRHISPROGLULEU
6   LYSVALGLUSERALAGLYLEUGLYALALEU
7   VALGLYLYSGLYALAASPPROTHRALAILE
8   SERVALALAALAGLUHISGLNLEUSERLEU
9   GLUGLYHISCYSALAARGGLNILESERARG
10   ARGLEUCYSARGASNTYRASPLEUILELEU
11   THRMETGLULYSARGHISILEGLUARGLEU
12   CYSGLUMETALAPROGLUMETARGGLYLYS
13   VALMETLEUPHEGLYHISTRPASPASNGLU
14   CYSGLUILEPROASPPROTYRARGLYSSER
15   ARGGLUTHRPHEALAALAVALTYRTHRLEU
16   LEUGLUARGSERALAARGGLNTRPALAGLN
17   ALALEUASNALAGLUGLNVAL

Samples:

sample_1: Wzb, [U-95% 13C; U-95% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 50 mM; DTT 25 mM; sodium azide 0.02%

sample_2: Wzb, [U-95% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 50 mM; DTT 25 mM; sodium azide 0.02%

conditions_1: pH: 6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N_HSQCnot availablenot availableconditions_1
1H-13C_HSQCnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
HN(CA)COnot availablenot availableconditions_1
CBCACONHnot availablenot availableconditions_1
HNCACBnot availablenot availableconditions_1
CCH-TOCSY-HSQCnot availablenot availableconditions_1
HCCH-TOCSY-HSQCnot availablenot availableconditions_1
CCH-COSY-HSQCnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ BAB36289 BAE76575 BAG11867 BAG11925 BAG11981
EMBL CAP76568 CAQ32473 CAQ89648 CAQ98984 CAR03445
GB AAC75122 AAC77834 AAG57121 AAN43662 AAP17490
REF NP_288567 NP_310893 NP_416565 NP_707955 NP_837681
SP P0AAB2 P0AAB3
AlphaFold P0AAB2 P0AAB3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks