BMRB Entry 6936

Name: Resonance assignments for the pKM101 homologue of VirB7 (TraN) in complex with the pKM101 homologue of VirB9 (TraO)
Published: 2006-04-14

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6936

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Resonance assignments for the pKM101 homologue of VirB7 (TraN) in complex with the pKM101 homologue of VirB9 (TraO) PubMed: 16601857

Deposition date: 2005-01-13 Original release date: 2006-04-14

Authors: Harris, Richard; Bayliss, Richard; Waksman, Gabriel; Driscoll, Paul

Citation: Harris, Richard; Bayliss, Richard; Waksman, Gabriel; Driscoll, Paul. "Resonance Assignments of the Complex between TraN and the C-terminal Domain of TraO from the Conjugative Plasmid pKM101" J. Biomol. NMR 36, 31-31 (2006).

Assembly members:
VirB7, polymer, 36 residues, 3772 Da.
VirB9, polymer, 126 residues, 14060 Da.

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
VirB7: GAMASGHKPPPEPDWSNTVP VNKTIPVDTQGGRNES
VirB9: GIDPFTHMAGAKNYQYVMSE QPEMRSIQPVHVWDNYRFTR FEFPANAELPQVYMISASGK ETLPNSHVVGENRNIIEVET VAKEWRIRLGDKVVGVRNNN FAPGAGAVATGTASPDVRRV QIGEDN

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	634
15N chemical shifts	151
1H chemical shifts	1016

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	VirB7	1
2	VirB9	2

Entities:

Entity 1, VirB7 36 residues - 3772 Da.

GAMA is part of the tag and no chemical shifts for the tag residues are reported. First VirB7 amino acid is S5 which in the proper sequence is residue number 17 as given in the chemical shift list.

1

GLY

ALA

MET

ALA

SER

GLY

HIS

LYS

PRO

2

PRO

GLU

PRO

ASP

TRP

SER

ASN

THR

VAL

PRO

3

VAL

ASN

LYS

THR

ILE

PRO

VAL

ASP

THR

GLN

4

GLY

ARG

ASN

GLU

SER

Entity 2, VirB9 126 residues - 14060 Da.

GIDPFTHM is part of the tag and no chemical shifts for the tag residues are reported. First VirB9 amino acid is A9 which in the proper sequence is residue number 177 as given in the chemical shift list.

1

GLY

ILE

ASP

PRO

PHE

THR

HIS

MET

ALA

GLY

2

ALA

LYS

ASN

TYR

GLN

TYR

VAL

MET

SER

GLU

3

GLN

PRO

GLU

MET

ARG

SER

ILE

GLN

PRO

VAL

4

HIS

VAL

TRP

ASP

ASN

TYR

ARG

PHE

THR

ARG

5

PHE

GLU

PHE

PRO

ALA

ASN

ALA

GLU

LEU

PRO

6

GLN

VAL

TYR

MET

ILE

SER

ALA

SER

GLY

LYS

7

GLU

THR

LEU

PRO

ASN

SER

HIS

VAL

GLY

8

GLU

ASN

ARG

ASN

ILE

GLU

VAL

GLU

THR

9

VAL

ALA

LYS

GLU

TRP

ARG

ILE

ARG

LEU

GLY

10

ASP

LYS

VAL

GLY

VAL

ARG

ASN

11

PHE

ALA

PRO

GLY

ALA

GLY

ALA

VAL

ALA

THR

12

GLY

THR

ALA

SER

PRO

ASP

VAL

ARG

VAL

13

GLN

ILE

GLY

GLU

ASP

ASN

Samples:

sample_1: VirB7, [U-15N], 1 mM; VirB9 1 mM

sample_2: VirB7, [U-13C; U-15N], 1 mM; VirB9 1 mM

sample_3: VirB7 1 mM; VirB9, [U-15N], 1 mM

sample_4: VirB7 1 mM; VirB9, [U-13C; U-15N], 1 mM

conditions_1: pH: 6.7; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H 15N HSQC	not available	not available	conditions_1
1H 13C HSQC	not available	not available	conditions_1
1H 13C CT-HSQC	not available	not available	conditions_1
1H 15N TOCSY-HSQC	not available	not available	conditions_1
1H 15N NOESY-HSQC	not available	not available	conditions_1
HNCO	not available	not available	conditions_1
HN(CA)CO	not available	not available	conditions_1
HNCA	not available	not available	conditions_1
HN(CO)CA	not available	not available	conditions_1
HNCACB	not available	not available	conditions_1
CBCA(CO)NH	not available	not available	conditions_1
HA(CA)NH	not available	not available	conditions_1
HA(CACO)NH	not available	not available	conditions_1
HCCH-TOCSY	not available	not available	conditions_1
HCCH-COSY	not available	not available	conditions_1
1H 13C NOESY-HSQC	not available	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Varian Unity Plus 500 MHz
Varian Inova 600 MHz
Varian Inova 800 MHz

PDB	2OFQ 3ZBI
DBJ	BAF38206 BAF93109 BAM29017
EMBL	CCA62524 CCE73953 CCN79904 CDF32038 CDK67343
GB	AAA86458 AAL13392 ABG33818 ABG49204 ABO42055
REF	NP_511196 WP_000735066 WP_000735067 WP_010999963 WP_013279390

Biological Magnetic Resonance Data Bank