BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6944

Title: Resonance assignment for the N-terminal region of eukaryotic initiation factor 5 (eIF5)   PubMed: 16738784

Deposition date: 2006-01-20 Original release date: 2006-08-11

Authors: Conte, Maria; Kelly, Geoff; Babon, Jeff; Proud, Christopher

Citation: Conte, Maria; Kelly, Geoff; Babon, Jeff; Proud, Christopher. "Resonance assignment for the N-terminal region of the eukaryotic initiation factor 5 (eIF5)"  J. Biomol. NMR 36, 42-42 (2006).

Assembly members:
Protein, polymer, 170 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Protein: LSVNVNRSVSDQFYRYKMPR LIAKVEGKGNGIKTVIVNMV DVAKALNRPPTYPTKYFGCE LGAQTQFDVKNDRYIVNGSH EANKLQDMLDGFIKKFVLCP ECENPETDLHVNPKKQTIGN SCKACGYRGMLDTHHKLCTF ILKNPPENSDSGTGKKEKEK KNRKGKDKEN

Data sets:
Data typeCount
13C chemical shifts567
15N chemical shifts148
1H chemical shifts806

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1eIF5(1-170)1
2Zinc ion2

Entities:

Entity 1, eIF5(1-170) 170 residues - Formula weight is not available

1   LEUSERVALASNVALASNARGSERVALSER
2   ASPGLNPHETYRARGTYRLYSMETPROARG
3   LEUILEALALYSVALGLUGLYLYSGLYASN
4   GLYILELYSTHRVALILEVALASNMETVAL
5   ASPVALALALYSALALEUASNARGPROPRO
6   THRTYRPROTHRLYSTYRPHEGLYCYSGLU
7   LEUGLYALAGLNTHRGLNPHEASPVALLYS
8   ASNASPARGTYRILEVALASNGLYSERHIS
9   GLUALAASNLYSLEUGLNASPMETLEUASP
10   GLYPHEILELYSLYSPHEVALLEUCYSPRO
11   GLUCYSGLUASNPROGLUTHRASPLEUHIS
12   VALASNPROLYSLYSGLNTHRILEGLYASN
13   SERCYSLYSALACYSGLYTYRARGGLYMET
14   LEUASPTHRHISHISLYSLEUCYSTHRPHE
15   ILELEULYSASNPROPROGLUASNSERASP
16   SERGLYTHRGLYLYSLYSGLULYSGLULYS
17   LYSASNARGLYSGLYLYSASPLYSGLUASN

Entity 2, Zinc ion - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: eIF5(1-170), [U-13C; U-15N], 0.5 mM; sodium phosphate 20 mM; DTT 2 mM

conditions_1: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
15N-and 13C edited NOESYsample_1not availableconditions_1
HNHAsample_1not availableconditions_1
15N-and 13C HSQCsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 800 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts