BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7011

Title: Solution structure of 97-109 segment of staphylococcal nuclease   PubMed: 16767771

Deposition date: 2006-02-28 Original release date: 2007-11-16

Authors: Wang, M.; Shan, L.; Wang, J.

Citation: Wang, M.; Shan, L.; Wang, J.. "Two peptide fragments G55-I72 and K97-A109 from staphylococcal nuclease exhibit different behaviors in conformational preferences for helix formation"  Biopolymers 83, 268-279 (2006).

Assembly members:
13-mer peptide from Thermonuclease (E.C.3.1.31.1), polymer, 13 residues, Formula weight is not available

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
13-mer peptide from Thermonuclease (E.C.3.1.31.1): KMVNEALVRQGLA

Data sets:
Data typeCount
13C chemical shifts43
1H chemical shifts76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
113-mer peptide from Thermonuclease1

Entities:

Entity 1, 13-mer peptide from Thermonuclease 13 residues - Formula weight is not available

1   LYSMETVALASNGLUALALEUVALARGGLN
2   GLYLEUALA

Samples:

sample_1: 13-mer peptide from Thermonuclease (E.C.3.1.31.1) 2.0 mM; DSS 0.02 mM; NaN3 0.02 mM; trifluoroethanol-d4 40%; H2O 60%

sample_cond_1: ionic strength: 0 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D ROESYnot availablenot availablenot available
2D 13C HSQCnot availablenot availablenot available
2D 13C HSQC-TOCSYnot availablenot availablenot available

Software:

xwinnmr v3.5 - collection

FELIX v98 - data analysis

CNS v1.1 - structure solution, refinement

NMR spectrometers:

  • Bruker DMX 600 MHz