BMRB Entry 7025

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7025

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Title:
1H, 13C, and 15N Resonance Assignments of the VAP-A: OSBP Complex
Deposition date:
2006-03-21
Original release date:
2006-10-02
Authors:
Furuita, Kyoko; Mishima, Masaki; Kojima, Chojiro
Citation:

Citation: Furuita, Kyoko; Mishima, Masaki; Kojima, Chojiro. "1H, 13C, and 15N Resonance Assignments of the VAP-A:OSBP Complex"  J. Biomol. NMR 36, 69-69 (2006).
PubMed: 17031525

Assembly members:

Assembly members:
vesicle-associated membrane protein-associated protein-A, polymer, 131 residues, 14812.0 Da.
oxisterol-binding protein, polymer, 48 residues, 5455.7 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
vesicle-associated membrane protein-associated protein-A: GPLGSDHEQILVLDPPTDLK FKGPFTDVVTTNLKLRNPSD RKVCFKVKTTAPRRYCVRPN SGIIDPGSTVTVSVMLQPFD YDPNEKSKHKFMVQTIFAPP NTSDMEAVWKEAKPDELMDS KLRCVFEMPNE
oxisterol-binding protein: GPLGSDHWGKGDMSDEDDEN EFFDAPEIITMPENLGHKRT GSHHHHHH

Data sets:
Data typeCount
13C chemical shifts652
15N chemical shifts160
1H chemical shifts1097

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1vesicle-associated membrane protein-associated protein-A1
2oxisterol-binding protein2

Entities:

Entity 1, vesicle-associated membrane protein-associated protein-A 131 residues - 14812.0 Da.

1   GLYPROLEUGLYSERASPHISGLUGLNILE
2   LEUVALLEUASPPROPROTHRASPLEULYS
3   PHELYSGLYPROPHETHRASPVALVALTHR
4   THRASNLEULYSLEUARGASNPROSERASP
5   ARGLYSVALCYSPHELYSVALLYSTHRTHR
6   ALAPROARGARGTYRCYSVALARGPROASN
7   SERGLYILEILEASPPROGLYSERTHRVAL
8   THRVALSERVALMETLEUGLNPROPHEASP
9   TYRASPPROASNGLULYSSERLYSHISLYS
10   PHEMETVALGLNTHRILEPHEALAPROPRO
11   ASNTHRSERASPMETGLUALAVALTRPLYS
12   GLUALALYSPROASPGLULEUMETASPSER
13   LYSLEUARGCYSVALPHEGLUMETPROASN
14   GLU

Entity 2, oxisterol-binding protein 48 residues - 5455.7 Da.

1   GLYPROLEUGLYSERASPHISTRPGLYLYS
2   GLYASPMETSERASPGLUASPASPGLUASN
3   GLUPHEPHEASPALAPROGLUILEILETHR
4   METPROGLUASNLEUGLYHISLYSARGTHR
5   GLYSERHISHISHISHISHISHIS

Samples:

sample_1: vesicle-associated membrane protein-associated protein-A, [U-13C; U-15N], 1.0 ± 0.2 mM; oxisterol-binding protein 1.0 ± 0.2 mM; K phosphate 50 mM; KCl 100 mM; DTT 1 mM; EDTA 0.1 mM

sample_2: vesicle-associated membrane protein-associated protein-A 1.0 ± 0.2 mM; oxisterol-binding protein, [U-13C; U15N], 1.0 ± 0.2 mM; K phosphate 50 mM; KCl 100 mM; DTT 1 mM; EDTA 0.1 mM

sample_3: vesicle-associated membrane protein-associated protein-A, [U-15N], 1.0 ± 0.2 mM; oxisterol-binding protein, [U-15N], 1.0 ± 0.2 mM; K phosphate 50 mM; KCl 100 mM; DTT 1 mM; EDTA 0.1 mM

conditions_1: pH: 6.9; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availableconditions_1
HN(CO)CACBnot availablenot availableconditions_1
HN(CA)COnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
H(CCO)NHnot availablenot availableconditions_1
C(CO)NHnot availablenot availableconditions_1
HC(CO)NHnot availablenot availableconditions_1
HCCH_TOCSYnot availablenot availableconditions_1
15N-edited TOCSY-HSQCnot availablenot availableconditions_1
15N-filtered 2D-NOESYnot availablenot availableconditions_1
1H15N HSQCnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker AVANCE 500 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
DBJ BAD90226 BAE21289
GB AAA31427 AAA59973 AAG17011 AAG28373 AAH11581
REF NP_001028346 NP_001075702 NP_001102397 NP_001253105 NP_002547
SP P16258 P22059 Q3B7Z2
AlphaFold P16258 P22059 Q3B7Z2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks