BMRB Entry 7072
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7072
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the PDZ domain of GOPC PubMed: 16882988
Deposition date: 2006-04-14 Original release date: 2006-04-24
Authors: Li, Xiang; Zhang, Jiahai; Wu, Jihui; Shi, Yunyu
Citation: Li, Xiang; Zhang, Jiahai; Cao, Zanxia; Wu, Jihui; Shi, Yunyu. "Solution Structure of GOPC PDZ Domain and Its Interaction with the C-Terminal motif of Neuroligin" Protein Sci. 15, 2149-2158 (2006).
Assembly members:
GOPC PDZ domain, polymer, 87 residues, Formula weight is not available
Natural source: Common Name: homo spaiens Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo spaiens
Experimental source: Production method: recombinant technology Vector: pET22(+)
Entity Sequences (FASTA):
GOPC PDZ domain: PIRKVLLLKEDHEGLGISIT
GGKEHGVPILISEIHPGQPA
DRCGGLHVGDAILAVNGVNL
RDTKHKEAVTILSQQRGEIE
FEVVYVA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 363 |
| 15N chemical shifts | 87 |
| 1H chemical shifts | 621 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GOPC PDZ monomer | 1 |
Entities:
Entity 1, GOPC PDZ monomer 87 residues - Formula weight is not available
| 1 | PRO | ILE | ARG | LYS | VAL | LEU | LEU | LEU | LYS | GLU | ||||
| 2 | ASP | HIS | GLU | GLY | LEU | GLY | ILE | SER | ILE | THR | ||||
| 3 | GLY | GLY | LYS | GLU | HIS | GLY | VAL | PRO | ILE | LEU | ||||
| 4 | ILE | SER | GLU | ILE | HIS | PRO | GLY | GLN | PRO | ALA | ||||
| 5 | ASP | ARG | CYS | GLY | GLY | LEU | HIS | VAL | GLY | ASP | ||||
| 6 | ALA | ILE | LEU | ALA | VAL | ASN | GLY | VAL | ASN | LEU | ||||
| 7 | ARG | ASP | THR | LYS | HIS | LYS | GLU | ALA | VAL | THR | ||||
| 8 | ILE | LEU | SER | GLN | GLN | ARG | GLY | GLU | ILE | GLU | ||||
| 9 | PHE | GLU | VAL | VAL | TYR | VAL | ALA |
Samples:
sample_1: GOPC PDZ domain, [U-15N], 1.0 ± 0.1 mM; phosphate buffer 20 ± 0.5 mM; EDTA 1 ± 0.1 mM; DTT 4 ± 0.1 mM; D2O 10 ± 0.5 %
sample_2: GOPC PDZ domain, [U-13C; U-15N], 1.0 ± 0.1 mM; phosphate buffer 20 ± 0.5 mM; EDTA 1 ± 0.1 mM; DTT 4 ± 0.1 mM; D2O 10 ± 0.5 %
conditions_1: pH: 6.0; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | not available | not available | conditions_1 |
| CBCACONH | not available | not available | conditions_1 |
| CBCANH | not available | not available | conditions_1 |
| HNCO | not available | not available | conditions_1 |
| HBHACBCACONH | not available | not available | conditions_1 |
| HCCONH-tocsy | not available | not available | conditions_1 |
| CCONH-tocsy | not available | not available | conditions_1 |
| 15N_NOESY | not available | not available | conditions_1 |
| HNCACO | not available | not available | conditions_1 |
| 13C_NOESY | not available | not available | conditions_1 |
| HCCH-tocsy | not available | not available | conditions_1 |
| HCCH-cosy | not available | not available | conditions_1 |
Software:
SPARKY v3 -
NMRPipe v2.3 -
NMR spectrometers:
- Bruker DMX 500 MHz
Related Database Links:
| BMRB | 18205 |
| PDB | |
| DBJ | BAB69946 BAC26958 BAC27058 BAC27951 |
| EMBL | CAH90325 |
| GB | AAG00571 AAG00572 AAH09553 AAH51171 AAI68160 |
| PRF | 1611455A |
| REF | NP_001017408 NP_001101101 NP_001125151 NP_001186201 NP_001193086 |
| SP | Q5RD32 Q8BH60 Q9HD26 |
| TPG | DAA26316 DAA26317 |
| AlphaFold | Q9HD26 Q5RD32 Q8BH60 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts