BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7099

Title: NMR Solution Structure of VP9 from White Spot Syndrome Virus   PubMed: 16956937

Deposition date: 2006-05-05 Original release date: 2008-07-16

Authors: Liu, Y.; Song, J.; Hew, C.

Citation: Liu, Y.; Wu, J.; Song, J.; Sivaraman, J.; Hew, C.. "Identification of a Novel Nonstructural Protein VP9 from White Spot Syndrome Virus: Its Structure Reveals a Ferredoxin Fold with Specific Metal Binding Sites."  J. Virol. 80, 10419-10427 (2006).

Assembly members:
wsv230, polymer, 85 residues, Formula weight is not available

Natural source:   Common Name: SHRIMP WHITE SPOT SYNDROME VIRUS   Taxonomy ID: 342409   Superkingdom: Viruses   Kingdom: not available   Genus/species: Whispovirus White spot syndrome virus 1

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
wsv230: GSHMATFQTDADFLLVGDDT SRYEEVMKTFDTVEAVRKSD LDDRVYMVCLKQGSTFVLNG GIEELRLLTGDSTLEIQPMI VPTTE

Data sets:
Data typeCount
13C chemical shifts240
15N chemical shifts80
1H chemical shifts507

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1wsv2301

Entities:

Entity 1, wsv230 85 residues - Formula weight is not available

1   GLYSERHISMETALATHRPHEGLNTHRASP
2   ALAASPPHELEULEUVALGLYASPASPTHR
3   SERARGTYRGLUGLUVALMETLYSTHRPHE
4   ASPTHRVALGLUALAVALARGLYSSERASP
5   LEUASPASPARGVALTYRMETVALCYSLEU
6   LYSGLNGLYSERTHRPHEVALLEUASNGLY
7   GLYILEGLUGLULEUARGLEULEUTHRGLY
8   ASPSERTHRLEUGLUILEGLNPROMETILE
9   VALPROTHRTHRGLU

Samples:

sample_1: wsv230 3 mM; phosphate buffer 20 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 20 mM; pH: 6.72; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HSQCnot availablenot availablenot available

Software:

NMRPipe v2004 - processing

CYANA v2.1 - refinement, structure solution

NMRView v5.2.2 - data analysis

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB AAK77784 AAL33234 AAL89153 ADR71658 AFX59607
REF NP_477752

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts