BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7129

Title: Solution Structure and Folding Characteristics of the C-Terminal SH3 Domain of c-Crk-II   PubMed: 16846230

Deposition date: 2006-05-23 Original release date: 2007-02-07

Authors: Dutta, Kaushik; Muralidharan, Vasant

Citation: Muralidharan, Vasant; Dutta, Kaushik; Cho, Jaehyun; Vila-Perello, Miquel; Raleigh, Daniel; Cowburn, David; Muir, Tom. "Solution Structure and Folding Characteristics of the C-Terminal SH3 Domain of c-Crk-II"  Biochemistry 45, 8874-8884 (2006).

Assembly members:
SH3 domain, polymer, 76 residues, Formula weight is not available

Natural source:   Common Name: E. Coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
SH3 domain: GLPNLQNGPIYARVIQKRVP NAYDKTALALEVGELVKVTK INVSGQWEGECNGKRGHFPF THVRLLDQQNPDEDFS

Data sets:
Data typeCount
13C chemical shifts314
15N chemical shifts80
1H chemical shifts460

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain1

Entities:

Entity 1, SH3 domain 76 residues - Formula weight is not available

1   GLYLEUPROASNLEUGLNASNGLYPROILE
2   TYRALAARGVALILEGLNLYSARGVALPRO
3   ASNALATYRASPLYSTHRALALEUALALEU
4   GLUVALGLYGLULEUVALLYSVALTHRLYS
5   ILEASNVALSERGLYGLNTRPGLUGLYGLU
6   CYSASNGLYLYSARGGLYHISPHEPROPHE
7   THRHISVALARGLEULEUASPGLNGLNASN
8   PROASPGLUASPPHESER

Samples:

Crk_SH3: SH3 domain, [U-100% 13C; U-100% 15N], 0.5 mM; Tris 10 mM; NaCl 50 mM; EDTA 1 mM

conditions_1: ionic strength: 50 mM; pH: 7.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCCrk_SH3not availableconditions_1
HNCOCrk_SH3not availableconditions_1
CBCACONHCrk_SH3not availableconditions_1
HNCACBCrk_SH3not availableconditions_1
CCCONHCrk_SH3not availableconditions_1
HBHACONHCrk_SH3not availableconditions_1
HCCONHCrk_SH3not availableconditions_1

Software:

NMRPipe -

NMRView v5.0.4, One Moon Scientific -

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAA01505 BAA07924 BAC25976 BAC36180 BAE32976
GB AAA49001 AAB30755 AAH01718 AAH08506 AAH12216
PIR I58394
REF NP_001007847 NP_001131108 NP_001179263 NP_058431 NP_062175
SP P46108 Q04929 Q63768 Q64010
TPG DAA19004
AlphaFold Q63768 P46108 Q04929 Q64010

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts