BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7172

Title: Backbone and side-chain 1H, 13C and 15N resonance assignments of C-terminal domain of an actin monomer binding protein twinfilin   PubMed: 16924408

Deposition date: 2006-06-16 Original release date: 2007-01-10

Authors: Hellman, Maarit; Paavilainen, Ville; Annila, Arto; Lappalainen, Pekka; Permi, Perttu

Citation: Hellman, Maarit; Paavilainen, Ville; Annila, Arto; Lappalainen, Pekka; Permi, Perttu. "NMR assignment of the C-terminal ADF-H domain of an actin monomer binding protein, twinfilin"  J. Biomol. NMR 36, 66-66 (2006).

Assembly members:
Twf-C, polymer, 142 residues, 16617 Da.

Natural source:   Common Name: House mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Vector: pRat4

Entity Sequences (FASTA):
Twf-C: AQGVAFPISRDAFQALEKLS KKQLNYVQLEIDIKNETIIL ANTENTELRDLPKRIPKDSA RYHFFLYKHSHEGDYLESVV FIYSMPGYTCSIRERMLYSS CKSPLLEIVERQLQMDVIRK IEIDNGDELTADFLYDEVHP KQ

Data sets:
Data typeCount
13C chemical shifts667
15N chemical shifts153
1H chemical shifts1057

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal domain of twinfilin1

Entities:

Entity 1, C-terminal domain of twinfilin 142 residues - 16617 Da.

1   ALAGLNGLYVALALAPHEPROILESERARG
2   ASPALAPHEGLNALALEUGLULYSLEUSER
3   LYSLYSGLNLEUASNTYRVALGLNLEUGLU
4   ILEASPILELYSASNGLUTHRILEILELEU
5   ALAASNTHRGLUASNTHRGLULEUARGASP
6   LEUPROLYSARGILEPROLYSASPSERALA
7   ARGTYRHISPHEPHELEUTYRLYSHISSER
8   HISGLUGLYASPTYRLEUGLUSERVALVAL
9   PHEILETYRSERMETPROGLYTYRTHRCYS
10   SERILEARGGLUARGMETLEUTYRSERSER
11   CYSLYSSERPROLEULEUGLUILEVALGLU
12   ARGGLNLEUGLNMETASPVALILEARGLYS
13   ILEGLUILEASPASNGLYASPGLULEUTHR
14   ALAASPPHELEUTYRASPGLUVALHISPRO
15   LYSGLN

Samples:

sample_1: C-terminal domain of twinfilin, [U-13C; U-15N], 1 mM; Bis-Tris 10 mM; Sodium Chloride 50 mM; Dithiothreitol 1 mM

conditions_1: ionic strength: 60 mM; pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCsample_1not availableconditions_1
13C-HSQCsample_1not availableconditions_1
13C-(CT)-HSQCsample_1not availableconditions_1
3D HNCAsample_1not availableconditions_1
3D HN(CO)CAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
HC(C)H-COSYsample_1not availableconditions_1
H(C)CH-TOCSYsample_1not availableconditions_1
(H)C(CO)NHsample_1not availableconditions_1
H(C)(CO)NHsample_1not availableconditions_1
(HB)CB(CGCDCE)HEsample_1not availableconditions_1
(HB)CB(CGCD)HDsample_1not availableconditions_1
DE-MQ-(H)CCmHm-TOCSYsample_1not availableconditions_1

Software:

VNMR v6.1 C - processing

NMR spectrometers:

  • Varian Inova 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts