BMRB Entry 7179
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7179
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Title: Dispersin Solution Structure PubMed: 17986189
Deposition date: 2006-06-21 Original release date: 2008-07-16
Authors: Velarde, J.; Varney, K.; Inman, K.; Weber, D.; Nataro, J.
Citation: Velarde, J.; Varney, K.; Farfan, M.; Dudley, E.; Inman, K.; Fletcher, J.; Weber, D.; Nataro, J.. "Solution structure of the novel dispersin protein of enteroaggregative Escherichia coli." Mol. Microbiol. 66, 1123-1135 (2007).
Assembly members:
Dispersin, Aap, polymer, 104 residues, Formula weight is not available
Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Dispersin, Aap: GGSGWNADNVDPSQCIKQSG
VQYTYNSGVSVCMQGLNEGK
VRGVSVSGVFYYNDGTTSNF
KGVVTPSTPVNTNQDINKTN
KVGVQKYRALTEWVGSRSHH
HHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 380 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 539 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Dispersin, Aap | 1 |
Entities:
Entity 1, Dispersin, Aap 104 residues - Formula weight is not available
| 1 | GLY | GLY | SER | GLY | TRP | ASN | ALA | ASP | ASN | VAL | ||||
| 2 | ASP | PRO | SER | GLN | CYS | ILE | LYS | GLN | SER | GLY | ||||
| 3 | VAL | GLN | TYR | THR | TYR | ASN | SER | GLY | VAL | SER | ||||
| 4 | VAL | CYS | MET | GLN | GLY | LEU | ASN | GLU | GLY | LYS | ||||
| 5 | VAL | ARG | GLY | VAL | SER | VAL | SER | GLY | VAL | PHE | ||||
| 6 | TYR | TYR | ASN | ASP | GLY | THR | THR | SER | ASN | PHE | ||||
| 7 | LYS | GLY | VAL | VAL | THR | PRO | SER | THR | PRO | VAL | ||||
| 8 | ASN | THR | ASN | GLN | ASP | ILE | ASN | LYS | THR | ASN | ||||
| 9 | LYS | VAL | GLY | VAL | GLN | LYS | TYR | ARG | ALA | LEU | ||||
| 10 | THR | GLU | TRP | VAL | GLY | SER | ARG | SER | HIS | HIS | ||||
| 11 | HIS | HIS | HIS | HIS |
Samples:
sample_1: Dispersin, Aap, [U-15N; U-13C], 1 mM; Phosphate 45 mM; NaCl 45 mM; EDTA 0.2 mM; NaN3 0.2 mM; DTT 0.1 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | not available | sample_cond_1 |
| 2D TOCSY | sample_1 | not available | sample_cond_1 |
| 3D 13C-separated NOESY | sample_1 | not available | sample_cond_1 |
| 3D 15N-separated NOESY | sample_1 | not available | sample_cond_1 |
| 4D 13C-separated NOESY | sample_1 | not available | sample_cond_1 |
| 4D 13C/15N-separated NOESY | sample_1 | not available | sample_cond_1 |
| 3D 15N-edited HOHAHA-HSQC | sample_1 | not available | sample_cond_1 |
| 3D 15N edited HMQC-NOESY-HSQC | sample_1 | not available | sample_cond_1 |
| 3D 15N HNHA | sample_1 | not available | sample_cond_1 |
| 3D HNCO | sample_1 | not available | sample_cond_1 |
| 3D C(CO)NH | sample_1 | not available | sample_cond_1 |
| 3D CBCA(CO)NH | sample_1 | not available | sample_cond_1 |
| 3D HNCACB | sample_1 | not available | sample_cond_1 |
| 3D HCA(CO)N | sample_1 | not available | sample_cond_1 |
| IPAP-HSQC | sample_1 | not available | sample_cond_1 |
| 3D HN(CO)CA | sample_1 | not available | sample_cond_1 |
Software:
X-PLOR NIH v2.9.1 - refinement
NMRPipe v1.8 - processing
xwinnmr v2.6 - collection
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts