BMRB Entry 7202
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7202
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for dSUMO
Deposition date: 2006-06-30 Original release date: 2007-07-02
Authors: Kumar, Ashutosh; Misra, Jyoti; Mittal, Rohit; Hosur, Ramakrishna
Citation: Kumar, Dinesh; Kumar, Ashutosh; Misra, Jyoti; Chugh, Jeetender; Sharma, Shilpy; Hosur, Ramakrishna. "1H, 15N, 13C resonance assignment of folded and 8 M urea-denatured state of SUMO from Drosophila melanogaster" Biomol. NMR Assignments 2, 13-15 (2008).
Assembly members:
dSUMO, polymer, 90 residues, 9972.2 Da.
Natural source: Common Name: Drosophila melanogaster Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
dSUMO: GSMSDEKKGGETEHINLKVL
GQDNAVVQFKIKKHTPLRKL
MNAYCDRAGLSMQVVRFRFD
GQPINENDTPTSLEMEEGDT
IEVYQQQTGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 356 |
| 15N chemical shifts | 90 |
| 1H chemical shifts | 564 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | dSUMO | 1 |
Entities:
Entity 1, dSUMO 90 residues - 9972.2 Da.
| 1 | GLY | SER | MET | SER | ASP | GLU | LYS | LYS | GLY | GLY | |
| 2 | GLU | THR | GLU | HIS | ILE | ASN | LEU | LYS | VAL | LEU | |
| 3 | GLY | GLN | ASP | ASN | ALA | VAL | VAL | GLN | PHE | LYS | |
| 4 | ILE | LYS | LYS | HIS | THR | PRO | LEU | ARG | LYS | LEU | |
| 5 | MET | ASN | ALA | TYR | CYS | ASP | ARG | ALA | GLY | LEU | |
| 6 | SER | MET | GLN | VAL | VAL | ARG | PHE | ARG | PHE | ASP | |
| 7 | GLY | GLN | PRO | ILE | ASN | GLU | ASN | ASP | THR | PRO | |
| 8 | THR | SER | LEU | GLU | MET | GLU | GLU | GLY | ASP | THR | |
| 9 | ILE | GLU | VAL | TYR | GLN | GLN | GLN | THR | GLY | GLY |
Samples:
sample_1: dSUMO, [U-13C; U-15N], 1 ± 0.1 mM; Sodium Phosphate 100 mM
conditions_1: pH: 5.6; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNN | sample_1 | not available | conditions_1 |
| HN(C)N | sample_1 | not available | conditions_1 |
| CBCACONH | sample_1 | not available | conditions_1 |
| CBCANH | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| TOCSY-HSQC | sample_1 | not available | conditions_1 |
| 15N-1H HSQC | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian Inova 600 MHz
Related Database Links:
| BMRB | 15473 |
| PDB | |
| EMBL | CBA18485 |
| GB | AAD19219 AAF31702 AAF52470 AAL28638 EAL32843 |
| REF | NP_477411 XP_001355784 XP_001650444 XP_001962391 XP_001970104 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts