BMRB Entry 7211
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7211
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Title: 1H and 15N Chemical Shift Assignments of Erabutoxin b
Deposition date: 2006-07-06 Original release date: 2009-05-28
Authors: Chuang, Woei-Jer; Chen, Chu-Chun
Citation: Chuang, Woei-Jer; Chen, Chu-Chun; Cheng, Chun-Ho; Chen, Yi-Chun; Chen, Chiu-Yueh. "1H and 15N Chemical Shifts of recombinant erabutoxin b" J. Biol. Chem. ., .-..
Assembly members:
erabutoxin b, polymer, 62 residues, Formula weight is not available
Natural source: Common Name: sea snake Taxonomy ID: 8631 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Laticauda semifasciata
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
erabutoxin b: RICFNHQSSQPQTTKTCSPG
ESSCYHKQWSDFRGTIIERG
CGCPTVKPGIKLSCCESEVC
NN
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 56 |
| 1H chemical shifts | 392 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | erabutoxin b monomer | 1 |
Entities:
Entity 1, erabutoxin b monomer 62 residues - Formula weight is not available
| 1 | ARG | ILE | CYS | PHE | ASN | HIS | GLN | SER | SER | GLN | ||||
| 2 | PRO | GLN | THR | THR | LYS | THR | CYS | SER | PRO | GLY | ||||
| 3 | GLU | SER | SER | CYS | TYR | HIS | LYS | GLN | TRP | SER | ||||
| 4 | ASP | PHE | ARG | GLY | THR | ILE | ILE | GLU | ARG | GLY | ||||
| 5 | CYS | GLY | CYS | PRO | THR | VAL | LYS | PRO | GLY | ILE | ||||
| 6 | LYS | LEU | SER | CYS | CYS | GLU | SER | GLU | VAL | CYS | ||||
| 7 | ASN | ASN |
Samples:
sample_1: erabutoxin b 3 ± 0.01 mM; H2O 90%; D2O 10%
sample_2: erabutoxin b 3 ± 0.01 mM; D2O 100%
sample_3: erabutoxin b, [U-98% 15N], 3 ± 0.01 mM; H2O 90%; D2O 10%
conditions_1: pH: 5.5; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
| 2D NOESY | sample_1 | not available | conditions_1 |
| 2D TOCSY | sample_1 | not available | conditions_1 |
| 2D DQF-COSY | sample_1 | not available | conditions_1 |
| 15N-edited NOESY | not available | not available | conditions_1 |
| 15N-edited TOCSY | not available | not available | conditions_1 |
Software:
xwinnmr v3.5, Bruker -
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA75749 BAA75750 BAA75751 BAA75752 BAC78199 |
| EMBL | CAA26373 CAA34824 CAA35770 |
| SP | P60775 Q7T2I5 Q90VW1 |
| AlphaFold | Q7T2I5 P60775 Q90VW1 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts