BMRB Entry 7250
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7250
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Title: Backbone 1H, 13C, 15N Chemical Shift Assignments for HIV-1 unmyristoylated matrix protein PubMed: 16840558
Deposition date: 2006-08-04 Original release date: 2006-08-15
Authors: Saad, Jamil; Miller, Jaime; Tai, Janet; Kim, Andrew; Ghanam, Ruba; Summers, Michael
Citation: Saad, Jamil; Miller, Jaime; Tai, Janet; Kim, Andrew; Ghanam, Ruba; Summers, Michael. "Structural Basis for targeting HIV-1 Gag proteins to the plasma membrane for virus assembly" Proc. Natl. Acad. Sci. U.S.A. 103, 11364-11369 (2006).
Assembly members:
Matrix protein, polymer, 131 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Matrix protein: GARASVLSGGELDKWEKIRL
RPGGKKQYKLKHIVWASREL
ERFAVNPGLLETSEGCRQIL
GQLQPSLQTGSEELRSLYNT
IAVLYCVHQRIDVKDTKEAL
DKIEEEQNKSKKKAQQAAAD
TGNNSQVSQNY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 429 |
| 15N chemical shifts | 131 |
| 1H chemical shifts | 782 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HIV-1 unmyristoylated Matrix | 1 |
Entities:
Entity 1, HIV-1 unmyristoylated Matrix 131 residues - Formula weight is not available
| 1 | GLY | ALA | ARG | ALA | SER | VAL | LEU | SER | GLY | GLY | ||||
| 2 | GLU | LEU | ASP | LYS | TRP | GLU | LYS | ILE | ARG | LEU | ||||
| 3 | ARG | PRO | GLY | GLY | LYS | LYS | GLN | TYR | LYS | LEU | ||||
| 4 | LYS | HIS | ILE | VAL | TRP | ALA | SER | ARG | GLU | LEU | ||||
| 5 | GLU | ARG | PHE | ALA | VAL | ASN | PRO | GLY | LEU | LEU | ||||
| 6 | GLU | THR | SER | GLU | GLY | CYS | ARG | GLN | ILE | LEU | ||||
| 7 | GLY | GLN | LEU | GLN | PRO | SER | LEU | GLN | THR | GLY | ||||
| 8 | SER | GLU | GLU | LEU | ARG | SER | LEU | TYR | ASN | THR | ||||
| 9 | ILE | ALA | VAL | LEU | TYR | CYS | VAL | HIS | GLN | ARG | ||||
| 10 | ILE | ASP | VAL | LYS | ASP | THR | LYS | GLU | ALA | LEU | ||||
| 11 | ASP | LYS | ILE | GLU | GLU | GLU | GLN | ASN | LYS | SER | ||||
| 12 | LYS | LYS | LYS | ALA | GLN | GLN | ALA | ALA | ALA | ASP | ||||
| 13 | THR | GLY | ASN | ASN | SER | GLN | VAL | SER | GLN | ASN | ||||
| 14 | TYR |
Samples:
sample_1: Matrix protein, [U-15 N; U-13 C], 0.5 mM
conditions_1: pH: 5.5; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | not available | conditions_1 |
| 3D 15N-edited NOESY | sample_1 | not available | conditions_1 |
| 13C-edited NOESY | sample_1 | not available | conditions_1 |
| 4D 15N,13C-edited NOESY | sample_1 | not available | conditions_1 |
| 4D 13C,13C-edited NOESY | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 800 MHz
Related Database Links:
| BMRB | 15114 15116 18715 18716 5316 5960 7267 7275 7309 |
| PDB | |
| DBJ | BAF34641 BAG48474 |
| EMBL | CBI61180 CBI61181 CBI61182 CBI61183 CBI61184 |
| GB | AAA44987 AAB00898 AAB60571 AAC28445 AAC29216 |
| SP | P12493 P12497 |
| AlphaFold | P12493 P12497 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts