BMRB Entry 7264

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7264

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone and Selected Sidechain 1H, 13C, and 15N Chemical Shift Assignments of the beta-1,4-glycosidase Cex from Cellulomonas fimi

Deposition date:

2006-08-15

Original release date:

2006-11-13

Authors:

Poon, David; Ludwiczek, Martin; Schubert, Mario; Withers, Stephen; McIntosh, Lawrence

Citation:

Citation: Poon, David; Ludwiczek, Martin; Schubert, Mario; Kwan, Emily; Withers, Stephen; McIntosh, Lawrence. "NMR spectroscopic characterization of a beta-(1,4)-glycosidase along its reaction pathway: stabilization upon formation of the glycosyl-enzyme intermediate." Biochemistry 46, 1759-1770 (2007).
PubMed: 17253772

Assembly members:

Assembly members:
apo-CexCD, polymer, 315 residues, 34230 Da.

Natural source:

Natural source: Common Name: Cellulomonas fimi Taxonomy ID: 1708 Superkingdom: Bacteria Kingdom: Actinobacteria Genus/species: Cellulomonas fimi

Experimental source:

Experimental source: Production method: recombinant technology Vector: pUC12-1.1Cex(PTIS)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
apo-CexCD: ATTLKEAADGAGRDFGFALD PNRLSEAQYKAIADSEFNLV VAENAMKWDATEPSQNSFSF GAGDRVASYAADTGKELYGH TLVWHSQLPDWAKNLNGSAF ESAMVNHVTKVADHFEGKVA SWDVVNEAFADGGGRRQDSA FQQKLGNGYIETAFRAARAA DPTAKLCINDYNVEGINAKS NSLYDLVKDFKARGVPLDCV GFQSHLIVGQVPGDFRQNLQ RFADLGVDVRITELDIRMRT PSDATKLATQAADYKKVVQA CMQVTRCQGVTVWGITDKYS WVPDVFPGEGAALVWDASYA KKPAYAAVMEAFGAS

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	939
15N chemical shifts	301
1H chemical shifts	306

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cex catalytic domain	1

Entities:

Entity 1, Cex catalytic domain 315 residues - 34230 Da.

1

ALA

THR

LEU

LYS

GLU

ALA

ASP

GLY

2

ALA

GLY

ARG

ASP

PHE

GLY

PHE

ALA

LEU

ASP

3

PRO

ASN

ARG

LEU

SER

GLU

ALA

GLN

TYR

LYS

4

ALA

ILE

ALA

ASP

SER

GLU

PHE

ASN

LEU

VAL

5

VAL

ALA

GLU

ASN

ALA

MET

LYS

TRP

ASP

ALA

6

THR

GLU

PRO

SER

GLN

ASN

SER

PHE

SER

PHE

7

GLY

ALA

GLY

ASP

ARG

VAL

ALA

SER

TYR

ALA

8

ALA

ASP

THR

GLY

LYS

GLU

LEU

TYR

GLY

HIS

9

THR

LEU

VAL

TRP

HIS

SER

GLN

LEU

PRO

ASP

10

TRP

ALA

LYS

ASN

LEU

ASN

GLY

SER

ALA

PHE

11

GLU

SER

ALA

MET

VAL

ASN

HIS

VAL

THR

LYS

12

VAL

ALA

ASP

HIS

PHE

GLU

GLY

LYS

VAL

ALA

13

SER

TRP

ASP

VAL

ASN

GLU

ALA

PHE

ALA

14

ASP

GLY

ARG

GLN

ASP

SER

ALA

15

PHE

GLN

LYS

LEU

GLY

ASN

GLY

TYR

ILE

16

GLU

THR

ALA

PHE

ARG

ALA

ARG

ALA

17

ASP

PRO

THR

ALA

LYS

LEU

CYS

ILE

ASN

ASP

18

TYR

ASN

VAL

GLU

GLY

ILE

ASN

ALA

LYS

SER

19

ASN

SER

LEU

TYR

ASP

LEU

VAL

LYS

ASP

PHE

20

LYS

ALA

ARG

GLY

VAL

PRO

LEU

ASP

CYS

VAL

21

GLY

PHE

GLN

SER

HIS

LEU

ILE

VAL

GLY

GLN

22

VAL

PRO

GLY

ASP

PHE

ARG

GLN

ASN

LEU

GLN

23

ARG

PHE

ALA

ASP

LEU

GLY

VAL

ASP

VAL

ARG

24

ILE

THR

GLU

LEU

ASP

ILE

ARG

MET

ARG

THR

25

PRO

SER

ASP

ALA

THR

LYS

LEU

ALA

THR

GLN

26

ALA

ASP

TYR

LYS

VAL

GLN

ALA

27

CYS

MET

GLN

VAL

THR

ARG

CYS

GLN

GLY

VAL

28

THR

VAL

TRP

GLY

ILE

THR

ASP

LYS

TYR

SER

29

TRP

VAL

PRO

ASP

VAL

PHE

PRO

GLY

GLU

GLY

30

ALA

LEU

VAL

TRP

ASP

ALA

SER

TYR

ALA

31

LYS

PRO

ALA

TYR

ALA

VAL

MET

GLU

32

ALA

PHE

GLY

ALA

SER

Samples:

apo_CexCD: apo-CexCD, [70% 2H; U-99% 13C; U-99% 15N], 0.4 mM; potassium phosphate 20 mM; sodium azide 0.02%

NMR_buffering_condition: pH: 6.5; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N TROSY HSQC	apo_CexCD	not available	NMR_buffering_condition
HNCA	apo_CexCD	not available	NMR_buffering_condition
HN(CO)CA	apo_CexCD	not available	NMR_buffering_condition
HNCO	apo_CexCD	not available	NMR_buffering_condition
HN(CA)CO	apo_CexCD	not available	NMR_buffering_condition
HN(CA)CB	apo_CexCD	not available	NMR_buffering_condition
HN(COCA)CB	apo_CexCD	not available	NMR_buffering_condition
NN-NOESY	apo_CexCD	not available	NMR_buffering_condition
HN-NOESY	apo_CexCD	not available	NMR_buffering_condition

Software:

NMRPipe -

NMR spectrometers:

Varian Inova 600 MHz

BMRB	7265
PDB	1EXP 1FH7 1FH8 1FH9 1FHD 1J01 2EXO 2HIS 2XYL 3CUF 3CUG 3CUH 3CUI 3CUJ
GB	AAA56791 AAA56792 AEA30147 AEE45406
PRF	1211268A
REF	WP_013770432 YP_004452793
SP	P07986
AlphaFold	P07986