BMRB Entry 7318

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7318

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Title:
Backbone 1HN, 15N, and 13C Chemical Shift Assignments for wt Im7* (* denotes his-tag) and its variants, Im7*L53AI54A and Im7*YY
Deposition date:
2006-10-17
Original release date:
2007-05-04
Authors:
Whittaker, Sara; Spence, Graham; Grossmann, Guenter; Radford, Sheena; Moore, Geoffrey
Citation:

Citation: Whittaker, Sara; Spence, Graham; Grossmann, Guenter; Radford, Sheena; Moore, Geoffrey. "NMR analysis of the conformational properties of the trapped on-pathway folding intermediate of the bacterial immunity protein Im7."  J. Mol. Biol. 366, 1001-1015 (2007).
PubMed: 17188712

Assembly members:

Assembly members:
YY, polymer, 94 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YY: MEHHHHHHELKNSISDYTEA EFVQLLKEIEKENVAATDDV LDVLLEHFVKITEHPDGGGG GYYPSDNRDDSPEGIVKEIK EWRAANGKPGFKQG

Data sets:
Data typeCount
13C chemical shifts202
15N chemical shifts68
1H chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Monomer1

Entities:

Entity 1, Monomer 94 residues - Formula weight is not available

The sequence of YY contains an N-terminal His-tag. Residue numbering starts at residue 2 of wt Im7 (no his-tag). Compared with Im7*, the residues T51-I54 have been mutated to Gly.

1   METGLUHISHISHISHISHISHISGLULEU
2   LYSASNSERILESERASPTYRTHRGLUALA
3   GLUPHEVALGLNLEULEULYSGLUILEGLU
4   LYSGLUASNVALALAALATHRASPASPVAL
5   LEUASPVALLEULEUGLUHISPHEVALLYS
6   ILETHRGLUHISPROASPGLYGLYGLYGLY
7   GLYTYRTYRPROSERASPASNARGASPASP
8   SERPROGLUGLYILEVALLYSGLUILELYS
9   GLUTRPARGALAALAASNGLYLYSPROGLY
10   PHELYSGLNGLY

Related Database Links:

GB EYB47026
REF WP_032277812

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks