BMRB Entry 7321

Name: Complete 1H 13C 15N chemical shift assignments for LDLa module from RXFP1
Published: 2006-11-06

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7321

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Complete 1H 13C 15N chemical shift assignments for LDLa module from RXFP1 PubMed: 17148455

Deposition date: 2006-10-22 Original release date: 2006-11-06

Authors: Hopkins, Emma; Gooley, Paul

Citation: Hopkins, Emma; Layfield, Sharon; Ferraro, Tania; Bathgate, Ross; Gooley, Paul. "The NMR solution structure of the relaxin (RXFP1) receptor lipoprotein receptor class A module and identification of key residues in the N-terminal region of the module that mediate receptor activation." J. Biol. Chem. 282, 4172-4184 (2007).

Assembly members:
LDLa, polymer, 43 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):
LDLa: GSQDVKCSLGYFPCGNITKC LPQLLHCNGVDDCGNQADED NCG

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	100
15N chemical shifts	43
1H chemical shifts	242

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LDLa module of RXFP1	1
2	CALCIUM (II) ION	2

Entities:

Entity 1, LDLa module of RXFP1 43 residues - Formula weight is not available

1

GLY

SER

GLN

ASP

VAL

LYS

CYS

SER

LEU

GLY

2

TYR

PHE

PRO

CYS

GLY

ASN

ILE

THR

LYS

CYS

3

LEU

PRO

GLN

LEU

HIS

CYS

ASN

GLY

VAL

4

ASP

CYS

GLY

ASN

GLN

ALA

ASP

GLU

ASP

5

ASN

CYS

GLY

Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.

1

CA

Samples:

sample_1: LDLa, [U-13C; U-15N], 1.5 ± 0.1 mM; soium acetate 50 mM; calcium chloride 10 mM; H2O 90%; D2O 10%

sample_2: LDLa, [U-15N], 1 ± 0.1 mM; soium acetate 50 mM; calcium chloride 10 mM; H2O 90%; D2O 10%

conditions_1: pH: 5.5; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N HSQC	sample_2	not available	conditions_1
CBCA(CO)NH	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
C(CO)NH	sample_1	not available	conditions_1
HC(CO)NH	sample_1	not available	conditions_1
HCCH-TOCSY	sample_1	not available	conditions_1
HNCO	sample_1	not available	conditions_1
HNHA	sample_2	not available	conditions_1
HNHB	sample_2	not available	conditions_1

Software:

NMRPipe v2.3, Frank Delaglio - processing

SPARKY v3.11, Goddard and Kneller - analysis

NMR spectrometers:

Varian Inova 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts