BMRB Entry 7358
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7358
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the golli myelin basic protein isoform BG21 PubMed: 17676872
Deposition date: 2007-01-10 Original release date: 2007-03-06
Authors: Ahmed, Mumdooh; Bamm, Vladimir; Harauz, George; Ladizhansky, Vladimir
Citation: Ahmed, Mumdooh; Bamm, Vladimir; Harauz, George; Ladizhansky, Vladimir. "The BG21 Isoform of Golli Myelin Basic Protein Is Intrinsically Disordered with a Highly Flexible Amino-Terminal Domain" Biochemistry 46, 9700-9712 (2007).
Assembly members:
recombinant murin BG21 isoform of golli myelin basic protein, polymer, 199 residues, 21611.5430 Da.
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Vector: pet22b=
Entity Sequences (FASTA):
recombinant murin BG21 isoform of golli myelin basic protein: GNHSGKRELSAEKASKDGEI
HRGEAGKKRSVGKLSQTASE
DSDVFGEADAIQNNGTSAED
TAVTDSKHTADPKNNWQGAH
PADPGNRPHLIRLFSRDAPG
REDNTFKDRPSESDELQTIQ
EDPTAASGGLDVMASQKRPS
QRSKYLATASTMDHARHGFL
PRHRDTGILDSIGRFFSGDR
GAPKRGSGKVSLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 546 |
| 15N chemical shifts | 192 |
| 1H chemical shifts | 353 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rmBG21 | 1 |
Entities:
Entity 1, rmBG21 199 residues - 21611.5430 Da.
| 1 | GLY | ASN | HIS | SER | GLY | LYS | ARG | GLU | LEU | SER | ||||
| 2 | ALA | GLU | LYS | ALA | SER | LYS | ASP | GLY | GLU | ILE | ||||
| 3 | HIS | ARG | GLY | GLU | ALA | GLY | LYS | LYS | ARG | SER | ||||
| 4 | VAL | GLY | LYS | LEU | SER | GLN | THR | ALA | SER | GLU | ||||
| 5 | ASP | SER | ASP | VAL | PHE | GLY | GLU | ALA | ASP | ALA | ||||
| 6 | ILE | GLN | ASN | ASN | GLY | THR | SER | ALA | GLU | ASP | ||||
| 7 | THR | ALA | VAL | THR | ASP | SER | LYS | HIS | THR | ALA | ||||
| 8 | ASP | PRO | LYS | ASN | ASN | TRP | GLN | GLY | ALA | HIS | ||||
| 9 | PRO | ALA | ASP | PRO | GLY | ASN | ARG | PRO | HIS | LEU | ||||
| 10 | ILE | ARG | LEU | PHE | SER | ARG | ASP | ALA | PRO | GLY | ||||
| 11 | ARG | GLU | ASP | ASN | THR | PHE | LYS | ASP | ARG | PRO | ||||
| 12 | SER | GLU | SER | ASP | GLU | LEU | GLN | THR | ILE | GLN | ||||
| 13 | GLU | ASP | PRO | THR | ALA | ALA | SER | GLY | GLY | LEU | ||||
| 14 | ASP | VAL | MET | ALA | SER | GLN | LYS | ARG | PRO | SER | ||||
| 15 | GLN | ARG | SER | LYS | TYR | LEU | ALA | THR | ALA | SER | ||||
| 16 | THR | MET | ASP | HIS | ALA | ARG | HIS | GLY | PHE | LEU | ||||
| 17 | PRO | ARG | HIS | ARG | ASP | THR | GLY | ILE | LEU | ASP | ||||
| 18 | SER | ILE | GLY | ARG | PHE | PHE | SER | GLY | ASP | ARG | ||||
| 19 | GLY | ALA | PRO | LYS | ARG | GLY | SER | GLY | LYS | VAL | ||||
| 20 | SER | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: recombinant murin BG21 isoform of golli myelin basic protein, [U-100% 13C; U-100% 15N], 1 mM; Potassium Chloride 100 mM; Sodium Azide 0.005%
conditions_1: pH: 6.8; temperature: 280 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| CBCA(CO)NH | sample_1 | not available | conditions_1 |
| HN(CA)CO | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| HACAN | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| DBJ | BAC30679 |
| EMBL | CAA47733 |
| GB | AAA37721 AAH51094 EDL09382 EDL09383 EDL09386 |
| REF | NP_001020416 XP_006526515 XP_006526516 XP_006526518 XP_006526520 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts