BMRB Entry 7371

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7371
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution NMR Structure: Northeast Structural Genomics Consortium Target SiR5
Deposition date:
2007-02-23
Original release date:
2010-03-09
Authors:
Wang, L.; Rossi, P.; Chen, C.; Nwosu, C.; Cunningham, K.; Ma, L.; Xiao, R.; Liu, J.; Baran, M.; Swapna, G.; Acton, T.; Burkhard, R.; Montelione, G.
Citation:

Citation: Wang, L.; Rossi, P.; Chen, C.; Nwosu, C.; Cunningham, K.; Ma, L.; Xiao, R.; Liu, J.; Baran, M.; Swapna, G.; Acton, T.; Burkhard, R.; Montelione, G.. "Northeast Structural Genomics Consortium Target SiR5"  To be Published ., .-..

Assembly members:

Assembly members:
SiR5, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 89184   Superkingdom: not available   Kingdom: Bacteria   Genus/species: Silicibacter Silicibacter

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SiR5: MMFLRKVEGPRSVTLPDGSI MTRADLPPANTRRWVASRKI AVVRGVIYGLITLAEAKQTY GLSDEEFNSWVSALAEHGKD ALKVTALKKYRQLLEHHHHH H

Data sets:
Data typeCount
13C chemical shifts420
15N chemical shifts93
1H chemical shifts682

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SiR51

Entities:

Entity 1, SiR5 101 residues - Formula weight is not available

1   METMETPHELEUARGLYSVALGLUGLYPRO
2   ARGSERVALTHRLEUPROASPGLYSERILE
3   METTHRARGALAASPLEUPROPROALAASN
4   THRARGARGTRPVALALASERARGLYSILE
5   ALAVALVALARGGLYVALILETYRGLYLEU
6   ILETHRLEUALAGLUALALYSGLNTHRTYR
7   GLYLEUSERASPGLUGLUPHEASNSERTRP
8   VALSERALALEUALAGLUHISGLYLYSASP
9   ALALEULYSVALTHRALALEULYSLYSTYR
10   ARGGLNLEULEUGLUHISHISHISHISHIS
11   HIS

Samples:

sample_1: SiR5, [U-13C; U-15N], 1.0 mM; NaCl 0.1 mM; D2O 5%; H2O 95%

sample_2: SiR5, [U-5% 13C; U-15N], 1.150 mM; NaCl 0.1 M; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D_13C-separated_NOESYnot availableisotropicsample_conditions_1
3D_15N-separated_NOESYnot availableisotropicsample_conditions_1
HCCH-TOCSYnot availableisotropicsample_conditions_1
HCCH-COSYnot availableisotropicsample_conditions_1
CccoNH-TOCSYnot availableisotropicsample_conditions_1
3D-HNCACBnot availableisotropicsample_conditions_1
HNcoCACBnot availableisotropicsample_conditions_1
HBHAcoNHnot availableisotropicsample_conditions_1
HNCAnot availableisotropicsample_conditions_1
HNCOnot availableisotropicsample_conditions_1
C13_HSQC_noct Stereospecific VL Me assignnot availableisotropicsample_conditions_1
Het-NOE, T1/Tirhonot availableisotropicsample_conditions_1
C13 Aromatic NOESYnot availableisotropicsample_conditions_1

Software:

VNMR v6.1C, Varian Inc. - collection

xwinnmr v3.5, Bruker Biospin - collection

DYANA v1.2 - structure solution

X-PLOR v2.11.2 - refinement

NMRPipe v2005, Delaglio - processing

AutoAssign v2.2.1, Moseley et al. - structure solution

AutoStruct v2.1.1, Huang et al. - structure solution

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAV94963
REF WP_011047413 YP_166917

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks