BMRB Entry 7375

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7375

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Title:
Backbone chemical shifts assignment of the soluble N-terminal region of the ATP7A
Deposition date:
2007-03-07
Original release date:
2007-10-24
Authors:
Banci, Lucia; Bertini, Ivano; Cantini, Francesca; Della Malva, Nunzia; Migliardi, Manuele; Rosato, Antonio
Citation:

Citation: Banci, Lucia; Bertini, Ivano; Cantini, Francesca; Della Malva, Nunzia; Migliardi, Manuele; Rosato, Antonio. "The different intermolecular interactions of the soluble copper-binding domains of the menkes protein, ATP7A.THE DIFFERENT INTERMOLECULAR INTERACTIONS OF THE SOLUBLE COPPER-BINDING DOMAINS OF THE MENKES PROTEIN, ATP7A"  J. Biol. Chem. 282, 23140-23146 (2007).
PubMed: 17545667

Assembly members:

Assembly members:
ATP7A, polymer, 629 residues, 68620.7 Da.
CU1, non-polymer, 63.546 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDEST17

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ATP7A: MGVNSVTISVEGMTCNSCVW TIEQQIGKVNGVHHIKVSLE EKNATIIYDPKLQTPKTLQE AIDDMGFDAVIHNPDPLPVL TDTLFLTVTASLTLPWDHIQ STLLKTKGVTDIKIYPQKRT VAVTIIPSIVNANQIKELVP ELSLDTGTLEKKSGACEDHS MAQAGEVVLKMKVEGMTCHS CTSTIEGKIGKLQGVQRIKV SLDNQEATIVYQPHLISVEE MKKQIEAMGFPAFVKKQPKY LKLGAIDVERLKNTPVKSSE GSQQRSPSYTNDSTATFIID GMHCKSCVSNIESTLSALQY VSSIVVSLENRSAIVKYNAS SVTPESLRKAIEAVSPGLYR VSITSEVESTSNSPSSSSLQ KIPLNVVSQPLTQETVINID GMTCNSCVQSIEGVISKKPG VKSIRVSLANSNGTVEYDPL LTSPETLRGAIEDMGFDATL SDTNEPLVVIAQPSSEMPLL TSTNEFYTKGMTPVQDKEEG KNSSKCYIQVTGMTCASCVA NIERNLRREEGIYSILVALM AGKAEVRYNPAVIQPPMIAE FIRELGFGATVIENADEGDG VLELVVRGMTCASCVHKIES SLTKHRGILYCSVALATNKA HIKYDPEIIGPRDIIHTIES LGFEASLVK

Data sets:
Data typeCount
13C chemical shifts390
15N chemical shifts373
1H chemical shifts372

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ATP7A1
2COPPER (I) ION2

Entities:

Entity 1, ATP7A 629 residues - 68620.7 Da.

1   METGLYVALASNSERVALTHRILESERVAL
2   GLUGLYMETTHRCYSASNSERCYSVALTRP
3   THRILEGLUGLNGLNILEGLYLYSVALASN
4   GLYVALHISHISILELYSVALSERLEUGLU
5   GLULYSASNALATHRILEILETYRASPPRO
6   LYSLEUGLNTHRPROLYSTHRLEUGLNGLU
7   ALAILEASPASPMETGLYPHEASPALAVAL
8   ILEHISASNPROASPPROLEUPROVALLEU
9   THRASPTHRLEUPHELEUTHRVALTHRALA
10   SERLEUTHRLEUPROTRPASPHISILEGLN
11   SERTHRLEULEULYSTHRLYSGLYVALTHR
12   ASPILELYSILETYRPROGLNLYSARGTHR
13   VALALAVALTHRILEILEPROSERILEVAL
14   ASNALAASNGLNILELYSGLULEUVALPRO
15   GLULEUSERLEUASPTHRGLYTHRLEUGLU
16   LYSLYSSERGLYALACYSGLUASPHISSER
17   METALAGLNALAGLYGLUVALVALLEULYS
18   METLYSVALGLUGLYMETTHRCYSHISSER
19   CYSTHRSERTHRILEGLUGLYLYSILEGLY
20   LYSLEUGLNGLYVALGLNARGILELYSVAL
21   SERLEUASPASNGLNGLUALATHRILEVAL
22   TYRGLNPROHISLEUILESERVALGLUGLU
23   METLYSLYSGLNILEGLUALAMETGLYPHE
24   PROALAPHEVALLYSLYSGLNPROLYSTYR
25   LEULYSLEUGLYALAILEASPVALGLUARG
26   LEULYSASNTHRPROVALLYSSERSERGLU
27   GLYSERGLNGLNARGSERPROSERTYRTHR
28   ASNASPSERTHRALATHRPHEILEILEASP
29   GLYMETHISCYSLYSSERCYSVALSERASN
30   ILEGLUSERTHRLEUSERALALEUGLNTYR
31   VALSERSERILEVALVALSERLEUGLUASN
32   ARGSERALAILEVALLYSTYRASNALASER
33   SERVALTHRPROGLUSERLEUARGLYSALA
34   ILEGLUALAVALSERPROGLYLEUTYRARG
35   VALSERILETHRSERGLUVALGLUSERTHR
36   SERASNSERPROSERSERSERSERLEUGLN
37   LYSILEPROLEUASNVALVALSERGLNPRO
38   LEUTHRGLNGLUTHRVALILEASNILEASP
39   GLYMETTHRCYSASNSERCYSVALGLNSER
40   ILEGLUGLYVALILESERLYSLYSPROGLY
41   VALLYSSERILEARGVALSERLEUALAASN
42   SERASNGLYTHRVALGLUTYRASPPROLEU
43   LEUTHRSERPROGLUTHRLEUARGGLYALA
44   ILEGLUASPMETGLYPHEASPALATHRLEU
45   SERASPTHRASNGLUPROLEUVALVALILE
46   ALAGLNPROSERSERGLUMETPROLEULEU
47   THRSERTHRASNGLUPHETYRTHRLYSGLY
48   METTHRPROVALGLNASPLYSGLUGLUGLY
49   LYSASNSERSERLYSCYSTYRILEGLNVAL
50   THRGLYMETTHRCYSALASERCYSVALALA
51   ASNILEGLUARGASNLEUARGARGGLUGLU
52   GLYILETYRSERILELEUVALALALEUMET
53   ALAGLYLYSALAGLUVALARGTYRASNPRO
54   ALAVALILEGLNPROPROMETILEALAGLU
55   PHEILEARGGLULEUGLYPHEGLYALATHR
56   VALILEGLUASNALAASPGLUGLYASPGLY
57   VALLEUGLULEUVALVALARGGLYMETTHR
58   CYSALASERCYSVALHISLYSILEGLUSER
59   SERLEUTHRLYSHISARGGLYILELEUTYR
60   CYSSERVALALALEUALATHRASNLYSALA
61   HISILELYSTYRASPPROGLUILEILEGLY
62   PROARGASPILEILEHISTHRILEGLUSER
63   LEUGLYPHEGLUALASERLEUVALLYS

Entity 2, COPPER (I) ION - Cu - 63.546 Da.

1   CU1

Samples:

sample_3: ATP7A, [U-99% 13C; U-99% 15N], 0.3 mM; DTT 1 mM; potassium phosphate 50 mM; tetrakis(acetonitrile)copper(I) hexafluorophosphate 1.8 mM

sample_4: ATP7A, [U-100% 15N], 0.7 mM; DTT 1 mM; potassium phosphate 50 mM; tetrakis(acetonitrile)copper(I) hexafluorophosphate 4.2 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_4isotropicsample_conditions_1
3D CBCA(CO)NHsample_4isotropicsample_conditions_1
3D HNCAsample_4isotropicsample_conditions_1
3D HN(CO)CAsample_4isotropicsample_conditions_1

Software:

CARA v1.5.3, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

BMRB 15164

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks