BMRB Entry 7381
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7381
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Title: Calcium binding protein in the free form
Deposition date: 2007-03-21 Original release date: 2015-10-27
Authors: Zhang, X.; Hu, Y.; Jin, C.
Citation: Zhang, X.; Hu, Y.; Xia, B.; Jin, C.. "Solution Structures of Ccbp from Anabaena Reveals a New Fold and Novel Calcium Binding Sites" To be Published ., .-..
Assembly members:
Alr1010_protein, polymer, 126 residues, Formula weight is not available
Natural source: Common Name: Anabaena sp. Taxonomy ID: 1167 Superkingdom: Bacteria Kingdom: not available Genus/species: Anabaena sp.
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Alr1010_protein: MASVERDETREHRIETEIIV
DAEDKEERAMGWYYYLDDTL
EFPFMGKWKKKSRKTSTIEE
KTVEVLGMAPDDECLKDMYV
EVADIGGKDDDVYTAKLSDI
EAIDVDDDTQEAIADWLYWL
ARGYKF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 532 |
| 15N chemical shifts | 127 |
| 1H chemical shifts | 805 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Alr1010_protein | 1 |
Entities:
Entity 1, Alr1010_protein 126 residues - Formula weight is not available
| 1 | MET | ALA | SER | VAL | GLU | ARG | ASP | GLU | THR | ARG | ||||
| 2 | GLU | HIS | ARG | ILE | GLU | THR | GLU | ILE | ILE | VAL | ||||
| 3 | ASP | ALA | GLU | ASP | LYS | GLU | GLU | ARG | ALA | MET | ||||
| 4 | GLY | TRP | TYR | TYR | TYR | LEU | ASP | ASP | THR | LEU | ||||
| 5 | GLU | PHE | PRO | PHE | MET | GLY | LYS | TRP | LYS | LYS | ||||
| 6 | LYS | SER | ARG | LYS | THR | SER | THR | ILE | GLU | GLU | ||||
| 7 | LYS | THR | VAL | GLU | VAL | LEU | GLY | MET | ALA | PRO | ||||
| 8 | ASP | ASP | GLU | CYS | LEU | LYS | ASP | MET | TYR | VAL | ||||
| 9 | GLU | VAL | ALA | ASP | ILE | GLY | GLY | LYS | ASP | ASP | ||||
| 10 | ASP | VAL | TYR | THR | ALA | LYS | LEU | SER | ASP | ILE | ||||
| 11 | GLU | ALA | ILE | ASP | VAL | ASP | ASP | ASP | THR | GLN | ||||
| 12 | GLU | ALA | ILE | ALA | ASP | TRP | LEU | TYR | TRP | LEU | ||||
| 13 | ALA | ARG | GLY | TYR | LYS | PHE |
Samples:
sample: Alr1010_protein, [U-13C; U-15N], 1 mM; Tris 20 mM; NaCl 220 mM; CaCl2 40 mM; DTT 10 mM; D2O 10%; H2O 90%
sample_conditions_1: pH: 7.5; pressure: 1 .; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D_13C-separated_NOESY | sample | isotropic | sample_conditions_1 |
| 3D_15N-separated_NOESY | sample | isotropic | sample_conditions_1 |
| HNCACB,CBCA(CO)NH | sample | isotropic | sample_conditions_1 |
| HNCO,HN(CA)CO | sample | isotropic | sample_conditions_1 |
| HC(C)H-COSY | sample | isotropic | sample_conditions_1 |
| HC(C)H-TOCSY | sample | isotropic | sample_conditions_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v2.1, F.Delaglio, S.Grzesiek, G.W.Vuister, G.Zhu, J.Pfeifer, A.Bax - processing
NMRView v5, B.A.Johnson, R.A.Blevins - data analysis
DYANA vcyana2.0, P.Guntert, C.Mumenthaler, W thrich, K.W.Thrich - structure solution
AMBER v7.0, D.A.Case - refinement
NMR spectrometers:
- Bruker AVANCE 800 MHz
- Bruker AVANCE 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts