Biological Magnetic Resonance Data Bank

A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7386

Title: Engrailed homeodomain helix-turn-helix motif   PubMed: 17517666

Deposition date: 2007-04-20 Original release date: 2007-10-29

Authors: Religa, T.

Citation: Religa, T.; Johnson, C.; Vu, D.; Brewer, S.; Dyer, R.; Fersht, A.. "The helix-turn-helix motif as an ultra-fast independently folding domain: The pathway of folding of Engrailed Homeodomain"  Proc. Natl. Acad. Sci. U. S. A. 104, 9272-9277 (2007).

Assembly members:
Segmentation_polarity_homeobox_protein_engrailed, polymer, 44 residues, Formula weight is not available

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Segmentation_polarity_homeobox_protein_engrailed: AKREFNENRYLTERRRQQLS SELGLNEAQIKIWFQNKRAK IKKS

Data sets:
Data typeCount
13C chemical shifts164
15N chemical shifts45
1H chemical shifts337
coupling constants86
residual dipolar couplings43

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Entity Assembly IDEntity NameEntity ID


Entity 1, homeobox 44 residues - Formula weight is not available



sample: homeobox 500 uM; d-acetate 50 mM; NaCl 100 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 145 mM; pH: 5.7; pressure: 1 atm; temperature: 278 K


NameSampleSample stateSample conditions
2D NOESYsampleisotropicsample_conditions_1
2D TOCSYsampleisotropicsample_conditions_1


NMRPipe vLast Update July 2, 2006, Delaglio - processing

SPARKY v3.106, Goddart - data analysis

CNS v1.1, Brunger - structure solution

CNS v1.1, Brunger - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts