BMRB Entry 932
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR932
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Title: Sequence-Specific 1H NMR Resonance Assignments of Bacillus subtilis HPr: Use of Spectra Obtained from Mutants To Resolve Spectral Overlap
Deposition date: 1995-07-31 Original release date: 1995-07-31
Authors: Wittekind, Michael; Reizer, Jonathan; Klevit, Rachel
Citation: Wittekind, Michael; Reizer, Jonathan; Klevit, Rachel. "Sequence-Specific 1H NMR Resonance Assignments of Bacillus subtilis HPr: Use of Spectra Obtained from Mutants To Resolve Spectral Overlap" Biochemistry 29, 7191-7200 (1990).
Assembly members:
phosphocarrier protein HPr, polymer, 87 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: not available Host organism: Escherichia coli
Entity Sequences (FASTA):
phosphocarrier protein HPr: AQKTFKVTADSGIHARPATV
LVQTASKYDADVNLEYNGKT
VNLKSIMGVMSLGIAKGAEI
TISASGADENDALNALEETM
KSEGLGQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 550 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | phosphocarrier protein HPr | 1 |
Entities:
Entity 1, phosphocarrier protein HPr 87 residues - Formula weight is not available
| 1 | ALA | GLN | LYS | THR | PHE | LYS | VAL | THR | ALA | ASP | ||||
| 2 | SER | GLY | ILE | HIS | ALA | ARG | PRO | ALA | THR | VAL | ||||
| 3 | LEU | VAL | GLN | THR | ALA | SER | LYS | TYR | ASP | ALA | ||||
| 4 | ASP | VAL | ASN | LEU | GLU | TYR | ASN | GLY | LYS | THR | ||||
| 5 | VAL | ASN | LEU | LYS | SER | ILE | MET | GLY | VAL | MET | ||||
| 6 | SER | LEU | GLY | ILE | ALA | LYS | GLY | ALA | GLU | ILE | ||||
| 7 | THR | ILE | SER | ALA | SER | GLY | ALA | ASP | GLU | ASN | ||||
| 8 | ASP | ALA | LEU | ASN | ALA | LEU | GLU | GLU | THR | MET | ||||
| 9 | LYS | SER | GLU | GLY | LEU | GLY | GLN |
Samples:
sample_one:
sample_condition_set_one: pH: 6.9 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_condition_set_one |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz