The algorithm, TATAPRO (Tracked Automated Assignments in Proteins) utilizes the protein primary sequence and peak lists from the following set of triple resonance spectra:

•     CBCANH
•     CBCA(CO)NH
•     HN(CA)CO (or HN(COCA)HA )
•     HNCO (or HN(CA)HA )

 

 
 
 
 

TATAPRO uses BMRB statistical data for ¹³C^a and ¹³C^b chemical shifts to group the 20 amino acids into 8 categories. It uses the above statistical data as a guide to correlate the protein primary sequence with the peak lists gerated from the above listed triple resonance experiments, thus correlating ¹H^N and ¹⁵N chemical shifts with those of ¹³C^a, ¹³C^b and ¹³C' and of the sequence neighbors.
 

To obtain a copy of TATAPRO (ANSI C code, can be compiled on UNIX or Windows workstations), please contact:

chary@tifr.res.in

J Biomol NMR. 2000 Jun;17(2):125-36.
[PubMed PMID: 10921777]

 
 
 
 
 
 

Protein	BMRB code	Mol. Wt.  (KDa)	No. of residues	No. of  mismatches	No. of Pro residues	Percentage of Assignments obtained on random deletion of peaks       0%           15%          30%
Calcium binding protein from Entamoeba histolytica	4271	15	134	3	0	96             85             77
Drosophila numb phosphotyrosine binding domain	4263	17.8	160	11	6	100             89            68
Fibroblast collagenase	4064	18.7	169	0	11	100             87            68
Borrelia burgdorferi OspA	4076	28	257	14	1	100             92            74
Escherichia coli maltose binding protein	4354	42	370	9	21	100             90            65