BMRB Entry 18704
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18704
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Title: human Siglec5 Carbohydrate Recognition Domain Chemical Shifts
Deposition date: 2012-09-06 Original release date: 2012-09-07
Authors: Barb, Adam; Prestegard, James
Citation: Barb, Adam; Wang, Xu; Prestegard, James. "NMR chemical shift mapping of refolded human sialic-acid-binding immunoglobulin-like lectin 5 binding to complex carbohydrates" .
Assembly members:
Siglec5, polymer, 143 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28b
Entity Sequences (FASTA):
Siglec5: MGSSHHHHHHSSGLVPRGSH
PVYELQVQKSVTVQEGLAVL
VPCSFSYPWRSWYSSPPLYV
YWFRDGEIPYYAEVVATNNP
DRRVKPETQGRFRLLGDVQK
KNCSLSIGDARMEDTGSYFF
RVERGRDVKYSYQQNKLNLE
VTA
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 330 |
15N chemical shifts | 110 |
1H chemical shifts | 110 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Siglec5 | 1 |
Entities:
Entity 1, Siglec5 143 residues - Formula weight is not available
This construct encodes residues P19 to A141 of human siglec5; from Uniprot DB: O15389
1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
3 | PRO | VAL | TYR | GLU | LEU | GLN | VAL | GLN | LYS | SER | ||||
4 | VAL | THR | VAL | GLN | GLU | GLY | LEU | ALA | VAL | LEU | ||||
5 | VAL | PRO | CYS | SER | PHE | SER | TYR | PRO | TRP | ARG | ||||
6 | SER | TRP | TYR | SER | SER | PRO | PRO | LEU | TYR | VAL | ||||
7 | TYR | TRP | PHE | ARG | ASP | GLY | GLU | ILE | PRO | TYR | ||||
8 | TYR | ALA | GLU | VAL | VAL | ALA | THR | ASN | ASN | PRO | ||||
9 | ASP | ARG | ARG | VAL | LYS | PRO | GLU | THR | GLN | GLY | ||||
10 | ARG | PHE | ARG | LEU | LEU | GLY | ASP | VAL | GLN | LYS | ||||
11 | LYS | ASN | CYS | SER | LEU | SER | ILE | GLY | ASP | ALA | ||||
12 | ARG | MET | GLU | ASP | THR | GLY | SER | TYR | PHE | PHE | ||||
13 | ARG | VAL | GLU | ARG | GLY | ARG | ASP | VAL | LYS | TYR | ||||
14 | SER | TYR | GLN | GLN | ASN | LYS | LEU | ASN | LEU | GLU | ||||
15 | VAL | THR | ALA |
Samples:
sample_1: Siglec5, [U-99% 13C; U-99% 15N], 0.2 0.4 mM; Arg 50 mM; Glu 50 mM; NaCl 150 mM; imidazole 10 mM
sample_2: Siglec5, [U-100% 13C; U-100% 15N; U-80% 2H], 0.2 mM; Arg 50 mM; Glu 50 mM; NaCl 150 mM; imidazole 10 mM
sample_conditions_1: ionic strength: 260 mM; pH: 6.5; pressure: 1 atm; temperature: 273 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
3D HN(COCA)CB | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
- Varian VXRS 900 MHz
Related Database Links:
PDB | |
DBJ | BAG60544 |
GB | AAB70703 AAD50978 AAF87846 AAH29896 AAK77223 |
REF | NP_001092082 NP_003821 XP_006723021 XP_011524598 XP_011524599 |
SP | O15389 Q08ET2 |
AlphaFold | O15389 Q08ET2 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts