BMRB Entry 10051

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR10051

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Title:
Backbone 1H and 15N assignment for the human c-Ha Ras (Y32W) in the GDP-bound state
Deposition date:
2006-11-13
Original release date:
2007-11-13
Authors:
Matsuda, T.; Koshiba, S.; Tochio, N.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Matsuda, T.; Koshiba, S.; Tochio, N.; Seki, E.; Iwasaki, N.; Yabuki, T.; Inoue, M.; Yokoyama, S.; Kigawa, T.. "Improving cell-free protein synthesis for stable-isotope labeling"  J. Biomol. NMR 37, 225-229 (2007).
PubMed: 17237976

Assembly members:

Assembly members:
RasY32W, polymer, 178 residues, 20034.82 Da.
GDP, non-polymer, 443.201 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RasY32W: GSSGSSGMTEYKLVVVGAGG VGKSALTIQLIQNHFVDEWD PTIEDSYRKQVVIDGETCLL DILDTAGQEEYSAMRDQYMR TGEGFLCVFAINNTKSFEDI HQYREQIKRVKDSDDVPMVL VGNKCDLAARTVESRQAQDL ARSYGIPYIETSAKTRQGVE DAFYTLVREIRQHKLRKL

Data sets:
Data typeCount
15N chemical shifts172
1H chemical shifts172

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RasY32W1
2GDP2

Entities:

Entity 1, RasY32W 178 residues - 20034.82 Da.

1   GLYSERSERGLYSERSERGLYMETTHRGLU
2   TYRLYSLEUVALVALVALGLYALAGLYGLY
3   VALGLYLYSSERALALEUTHRILEGLNLEU
4   ILEGLNASNHISPHEVALASPGLUTRPASP
5   PROTHRILEGLUASPSERTYRARGLYSGLN
6   VALVALILEASPGLYGLUTHRCYSLEULEU
7   ASPILELEUASPTHRALAGLYGLNGLUGLU
8   TYRSERALAMETARGASPGLNTYRMETARG
9   THRGLYGLUGLYPHELEUCYSVALPHEALA
10   ILEASNASNTHRLYSSERPHEGLUASPILE
11   HISGLNTYRARGGLUGLNILELYSARGVAL
12   LYSASPSERASPASPVALPROMETVALLEU
13   VALGLYASNLYSCYSASPLEUALAALAARG
14   THRVALGLUSERARGGLNALAGLNASPLEU
15   ALAARGSERTYRGLYILEPROTYRILEGLU
16   THRSERALALYSTHRARGGLNGLYVALGLU
17   ASPALAPHETYRTHRLEUVALARGGLUILE
18   ARGGLNHISLYSLEUARGLYSLEU

Entity 2, GDP - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

Related Database Links:

BMRB 17183 17610 17678 18461 18479 18629 25730
PDB 121P 1AA9 1AGP 1BKD 1CLU 1CRP 1CRQ 1CRR 1CTQ 1GNP 1GNQ 1GNR 1HE8 1IAQ 1IOZ 1JAH 1JAI 1K8R 1LF0 1LF5 1LFD 1NVU 1NVV 1NVW 1NVX 1P2S 1P2T 1P2U 1P2V 1PLJ 1PLK 1PLL 1Q21 1QRA 1RVD 1WQ1 1XCM 1XD2 1XJ0 1ZVQ 1ZW6 221P 2C5L 2CE2 2CL0 2CL6 2CL7 2CLC 2CLD 2EVW 2LCF 2LWI 2N42 2N46 2Q21 2QUZ 2RGA 2RGB 2RGC 2RGD 2RGE 2RGG 2UZI 2VH5 2X1V 3DDC 3I3S 3K8Y 3K9L 3K9N 3KKM 3KKN 3KUD 3L8Y 3L8Z 3LBH 3LBI 3LBN 3LO5 3OIU 3OIV 3OIW 3RRY 3RRZ 3RS0 3RS2 3RS3 3RS4 3RS5 3RS7 3RSL 3RSO 3TGP 3V4F 421P 4DLR 4DLS 4DLT 4DLU 4DLV 4DLW 4DLX 4DLY 4DLZ 4EFL 4G0N 4G3X 4NYI 4NYJ 4NYM 4Q21 4RSG 4URU 4URV 4URW 4URX 4URY 4URZ 4US0 4US1 4US2 4XVQ 4XVR 521P 5P21 621P 6Q21 721P 821P
DBJ BAB61869 BAB61870 BAB88314 BAB88315 BAB88316
EMBL CAA25322 CAA25624 CAA27258 CAA90306 CAD24594
GB AAA42009 AAA46568 AAA46569 AAA46570 AAA46574
PIR A43816 TVMVNS
PRF 0904302A 1604384A
REF NP_001017003 NP_001018465 NP_001084278 NP_001091711 NP_001123913
SP P01112 P01113 P01114 P01115 P08642
TPG DAA13500
AlphaFold P01112 P01113 P01114 P01115 P08642

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks