BMRB Entry 6306

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6306

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Title:
Mainchain proton and nitrogen chemical shifts for the C-domain of human RanGAP1 linked via an isopeptide at Lys524 to Gly97 of human Sumo-1
Deposition date:
2004-08-27
Original release date:
2004-11-29
Authors:
Macauley, Matthew; Errington, Wesley; Okon, Mark; Scharpf, Manuela; Mackereth, Cameron; Schulman, Brenda; McIntosh, Lawrence
Citation:

Citation: Macauley, Matthew; Errington, Wesley; Okon, Mark; Scharpf, Manuela; Mackereth, Cameron; Schulman, Brenda; McIntosh, Lawrence. "Structural and Dynamic Independence of Isopeptide-linked RanGAP1 and SUMO-1"  J. Biol. Chem. 279, 49131-49137 (2004).
PubMed: 15355965

Assembly members:

Assembly members:
Sumoylated RanGAP1, polymer, 172 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Animalia   Genus/species: Homo sapian

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sumoylated RanGAP1: GSHMGEPAPVLSSPPPADVS TFLAFPSPEKLLRLGPKSSV LIAQQTDTSDPEKVVSAFLK VSSVFKDEATVRMAVQDAVD ALMQKAFNSSSFNSNTFLTR LLVHMGLLKSEDKVKAIANL YGPLMALNHMVQQDYFPKAL APLLLAFVTKPNSALESCSF ARHSLLQTLYKV

Data sets:
Data typeCount
1H chemical shifts156
15N chemical shifts154

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Sumoylated RanGAP11

Entities:

Entity 1, Sumoylated RanGAP1 172 residues - Formula weight is not available

1   GLYSERHISMETGLYGLUPROALAPROVAL
2   LEUSERSERPROPROPROALAASPVALSER
3   THRPHELEUALAPHEPROSERPROGLULYS
4   LEULEUARGLEUGLYPROLYSSERSERVAL
5   LEUILEALAGLNGLNTHRASPTHRSERASP
6   PROGLULYSVALVALSERALAPHELEULYS
7   VALSERSERVALPHELYSASPGLUALATHR
8   VALARGMETALAVALGLNASPALAVALASP
9   ALALEUMETGLNLYSALAPHEASNSERSER
10   SERPHEASNSERASNTHRPHELEUTHRARG
11   LEULEUVALHISMETGLYLEULEULYSSER
12   GLUASPLYSVALLYSALAILEALAASNLEU
13   TYRGLYPROLEUMETALALEUASNHISMET
14   VALGLNGLNASPTYRPHEPROLYSALALEU
15   ALAPROLEULEULEUALAPHEVALTHRLYS
16   PROASNSERALALEUGLUSERCYSSERPHE
17   ALAARGHISSERLEULEUGLNTHRLEUTYR
18   LYSVAL

Related Database Links:

BMRB 6305
PDB 1Z5S 2GRN 2GRO 2GRP 2GRQ 2GRR 2IO2 2IO3 2IY0 3UIN 3UIO 3UIP
DBJ BAB47464 BAG10161 BAG63578 BAG64552
EMBL CAA57714 CAG30443 CAK54588 CAK54887
GB AAH04990 AAH14044 AAH41396 AIC55014 EAW60420
REF NP_001265580 NP_002874 XP_004063574 XP_004063575 XP_004063576
SP P46060
AlphaFold P46060

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks