BMRB Entry 7307

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7307

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Title:
Backbone assignment and RDCs of L11 in complex with RNA
Deposition date:
2006-09-28
Original release date:
2006-12-20
Authors:
Jonker, Henry; Ilin, Serge; Grimm, Steffen; Wohnert, Jens; Schwalbe, Harald
Citation:

Citation: Jonker, Henry; Ilin, Serge; Grimm, Steffen; Wohnert, Jens; Schwalbe, Harald. "L11 domain rearrangement upon binding to RNA and thiostrepton studied by NMR spectroscopy."  Nucleic Acids Res. 35, 441-454 (2007).
PubMed: 17169991

Assembly members:

Assembly members:
ribosomal L11 protein, polymer, 141 residues, 15088 Da.
23S GAR RNA, polymer, 60 residues, 19258 Da.

Natural source:

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ribosomal L11 protein: MAKKVAAQIKLQLPAGKATP APPVGPALGQHGVNIMEFCK RFNAETADKAGMILPVVITV YEDKSFTFIIKTPPASFLLK KAAGIEKGSSEPKRKIVGKV TRKQIEEIAKTKMPDLNANS LEAAMKIIEGTAKSMGIEVV D
23S GAR RNA: GGGCAGGAUGUAGGCUUAGA AGCAGCCAUCAUUUAAAGAA AGCGUAAUAGCUCACUGCCC

Data sets:
Data typeCount
13C chemical shifts256
15N chemical shifts127
1H chemical shifts127
residual dipolar couplings127

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1L11 protein1
2GAR RNA2

Entities:

Entity 1, L11 protein 141 residues - 15088 Da.

1-141, Thermotoga maritima

1   METALALYSLYSVALALAALAGLNILELYS
2   LEUGLNLEUPROALAGLYLYSALATHRPRO
3   ALAPROPROVALGLYPROALALEUGLYGLN
4   HISGLYVALASNILEMETGLUPHECYSLYS
5   ARGPHEASNALAGLUTHRALAASPLYSALA
6   GLYMETILELEUPROVALVALILETHRVAL
7   TYRGLUASPLYSSERPHETHRPHEILEILE
8   LYSTHRPROPROALASERPHELEULEULYS
9   LYSALAALAGLYILEGLULYSGLYSERSER
10   GLUPROLYSARGLYSILEVALGLYLYSVAL
11   THRARGLYSGLNILEGLUGLUILEALALYS
12   THRLYSMETPROASPLEUASNALAASNSER
13   LEUGLUALAALAMETLYSILEILEGLUGLY
14   THRALALYSSERMETGLYILEGLUVALVAL
15   ASP

Entity 2, GAR RNA 60 residues - 19258 Da.

1050-1109, based on Escherichia coli 23S rRNA (U1061A)

1   GGGCAGGAUG
2   UAGGCUUAGA
3   AGCAGCCAUC
4   AUUUAAAGAA
5   AGCGUAAUAG
6   CUCACUGCCC

Related Database Links:

BMRB 5513
PDB 1EG0 1GIY 1JQM 1JQS 1JQT 1MJ1 1ML5 1MMS 1MVR 1OLN 1PN7 1PN8 1R2W 1R2X 1YL3 2B66 2B9N 2B9P 2JQ7 2K3F 487D
EMBL CAA77859
GB AAD35537 ABQ46490 ACB08837 ADA66341 AGL49376
REF NP_228264 WP_004081512 WP_011943108 WP_038033608 YP_001244066
SP A5IJW7 B1L939 P29395
AlphaFold A5IJW7 B1L939 P29395

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks