BMRB Entry 15995

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PDB ID: 2k9p
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15995
MolProbity Validation Chart

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Title:
Structure of TM1_TM2 in LPPG micelles
Deposition date:
2008-10-21
Original release date:
2009-05-18
Authors:
Neumoin, Alexey; Zerbe, Oliver; Fred, Naider
Citation:

Citation: Neumoin, Alexey; Cohen, Leah; Arshava, Boris; Tantry, Subramanyam; Becker, Jeffey; Zerbe, Oliver; Naider, Fred. "STRUCTURE OF A DOUBLE TRANSMEMBRANE FRAGMENT OF A G PROTEIN-COUPLED RECEPTOR IN MICELLES"  Biophys J. 96, 3187-3196 (2009).
PubMed: 19383463

Assembly members:

Assembly members:
TM1_TM2 in LPPG micelles, polymer, 80 residues, 8757.232 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLC01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TM1_TM2 in LPPG micelles: GNGSTITFDELQGLVNSTVT QAILFGVRSGAAALTLIVVW ITSRSRKTPIFIINQVSLFL IILHSALYFKYLLSNYSSVT

Data sets:
Data typeCount
13C chemical shifts350
15N chemical shifts76
1H chemical shifts533

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1TM1_TM2 in LPPG micelles1

Entities:

Entity 1, TM1_TM2 in LPPG micelles 80 residues - 8757.232 Da.

fragment comprising part of the N-terminal domain, TM1, I1, TM2, E1

1   GLYASNGLYSERTHRILETHRPHEASPGLU
2   LEUGLNGLYLEUVALASNSERTHRVALTHR
3   GLNALAILELEUPHEGLYVALARGSERGLY
4   ALAALAALALEUTHRLEUILEVALVALTRP
5   ILETHRSERARGSERARGLYSTHRPROILE
6   PHEILEILEASNGLNVALSERLEUPHELEU
7   ILEILELEUHISSERALALEUTYRPHELYS
8   TYRLEULEUSERASNTYRSERSERVALTHR

Related Database Links:

BMRB 17211 17593
PDB 2K9P

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