BMRB Entry 17641

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17641

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Title:
Assigment of the 1H, 13C, and 15N resonances of the yeast frataxin (Yfh1) under heat denaturation (50 C)
Deposition date:
2011-05-12
Original release date:
2015-09-03
Authors:
Adrover, Miquel; Pastore, Annalisa; Temussi, Piero Andrea
Citation:

Citation: Adrover, Miquel; Martorell, Gabriel; Martin, Stephen; Urosev, Dunja; Konarev, Petr; Svergun, Dmitri; Daura, Xavier; Temussi, Pierandrea; Pastore, Annalisa. "The role of hydration in protein stability: comparison of the cold and heat unfolded states of Yfh1."  J. Mol. Biol. 417, 413-424 (2012).
PubMed: 22342930

Assembly members:

Assembly members:
Yfh1, polymer, 123 residues, 13783.4 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Yfh1: MESSTDGQVVPQEVLNLPLE KYHEEADDYLDHLLDSLEEL SEAHPDCIPDVELSHGVMTL EIPAFGTYVINKQPPNKQIW LASPLSGPNRFDLLNGEWVS LRNGTKLTDILTEEVEKAIS KSQ

Data sets:
Data typeCount
13C chemical shifts236
15N chemical shifts104
1H chemical shifts535

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Yeast Frataxin1

Entities:

Entity 1, Yeast Frataxin 123 residues - 13783.4 Da.

The sequence does not present any tag neither any additional residue. It is in its native state

1   METGLUSERSERTHRASPGLYGLNVALVAL
2   PROGLNGLUVALLEUASNLEUPROLEUGLU
3   LYSTYRHISGLUGLUALAASPASPTYRLEU
4   ASPHISLEULEUASPSERLEUGLUGLULEU
5   SERGLUALAHISPROASPCYSILEPROASP
6   VALGLULEUSERHISGLYVALMETTHRLEU
7   GLUILEPROALAPHEGLYTHRTYRVALILE
8   ASNLYSGLNPROPROASNLYSGLNILETRP
9   LEUALASERPROLEUSERGLYPROASNARG
10   PHEASPLEULEUASNGLYGLUTRPVALSER
11   LEUARGASNGLYTHRLYSLEUTHRASPILE
12   LEUTHRGLUGLUVALGLULYSALAILESER
13   LYSSERGLN

Related Database Links:

BMRB 17068 6356 17068 19991
PDB 1soy 2fql 2ga5 2FQL 2GA5 3OEQ 3OER 4EC2
DBJ GAA22128
EMBL CAA98688 CAY78388
GB AAS56486 AHY74893 AJP37633 AJU57746 AJU58449
REF NP_010163
SP Q07540
TPG DAA11740
AlphaFold Q07540

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SPARKY: Backbone or all simulated peaks