BMRB Entry 17879
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PDB ID: 2li5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17879
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Noda, Nobuo; Satoo, Kenji; Fujioka, Yuko; Kumeta, Hiroyuki; Ogura, Kenji; Nakatogawa, Hitoshi; Ohsumi, Yoshinori; Inagaki, Fuyuhiko. "Structural basis of Atg8 activation and transfer to Atg3 by a homodimeric E1, Atg7" Mol. Cell 44, 462-475 (2011).
PubMed: 22055191
Assembly members:
Atg8K26P, polymer, 117 residues, 13597.823 Da.
ATG7C30, polymer, 34 residues, 3848.176 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Aspergillus niger Vector: pGEX6p
Entity Sequences (FASTA):
Atg8K26P: HMKSTFKSEYPFEKRKAESE
RIADRFPNRIPVICEKAEKS
DIPEIDKRKYLVPADLTVGQ
FVYVIRKRIMLPPEKAIFIF
VNDTLPPTAALMSAIYQEHK
DKDGFLYVTYSGENTFG
ATG7C30: GPHMISGLSVIKQEVERLGN
DVFEWEDDESDEIA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 681 |
| 15N chemical shifts | 154 |
| 1H chemical shifts | 1111 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Atg8K26P | 1 |
| 2 | ATG7C30 | 2 |
Entities:
Entity 1, Atg8K26P 117 residues - 13597.823 Da.
| 1 | HIS | MET | LYS | SER | THR | PHE | LYS | SER | GLU | TYR | ||||
| 2 | PRO | PHE | GLU | LYS | ARG | LYS | ALA | GLU | SER | GLU | ||||
| 3 | ARG | ILE | ALA | ASP | ARG | PHE | PRO | ASN | ARG | ILE | ||||
| 4 | PRO | VAL | ILE | CYS | GLU | LYS | ALA | GLU | LYS | SER | ||||
| 5 | ASP | ILE | PRO | GLU | ILE | ASP | LYS | ARG | LYS | TYR | ||||
| 6 | LEU | VAL | PRO | ALA | ASP | LEU | THR | VAL | GLY | GLN | ||||
| 7 | PHE | VAL | TYR | VAL | ILE | ARG | LYS | ARG | ILE | MET | ||||
| 8 | LEU | PRO | PRO | GLU | LYS | ALA | ILE | PHE | ILE | PHE | ||||
| 9 | VAL | ASN | ASP | THR | LEU | PRO | PRO | THR | ALA | ALA | ||||
| 10 | LEU | MET | SER | ALA | ILE | TYR | GLN | GLU | HIS | LYS | ||||
| 11 | ASP | LYS | ASP | GLY | PHE | LEU | TYR | VAL | THR | TYR | ||||
| 12 | SER | GLY | GLU | ASN | THR | PHE | GLY |
Entity 2, ATG7C30 34 residues - 3848.176 Da.
| 1 | GLY | PRO | HIS | MET | ILE | SER | GLY | LEU | SER | VAL | ||||
| 2 | ILE | LYS | GLN | GLU | VAL | GLU | ARG | LEU | GLY | ASN | ||||
| 3 | ASP | VAL | PHE | GLU | TRP | GLU | ASP | ASP | GLU | SER | ||||
| 4 | ASP | GLU | ILE | ALA |
Related Database Links:
| BMRB | 16120 16835 |
| PDB | 2KQ7 2KWC 2LI5 2ZPN 3RUI 3VH3 3VH4 3VXW 3T7E |
| DBJ | GAA21477 BAA33474 |
| EMBL | CAA56032 CAA84899 CAY77706 |
| GB | AAT92889 AHY74413 AJP37015 AJP81917 AJP82297 AAB68016 EIW10078 EWH18191 |
| REF | NP_009475 NP_012041 |
| SP | A6ZKM4 P38182 P38862 |
| TPG | DAA07045 DAA06864 |
| AlphaFold | A6ZKM4 P38182 P38862 |
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