BMRB Entry 18919

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Full-Length Rabbit Cytochrome b5
Published: 2013-02-05

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PDB ID: 2m33
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18919
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Full-Length Rabbit Cytochrome b5

Deposition date:

2012-12-26

Original release date:

2013-02-05

Authors:

Subramanian, Vivekanandan; Ahuja, Shivani; Popovych, Nataliya; Huang, Rui; Le Clair, Stephanie; Jahr, Nicole; Soong, Ronald; Xu, Jiadi; Yamamoto, Kazutoshi; Nanga, Ravi; Im, Sang-Choul; Waskell, Lucy; Ramamoorthy, Ayyalusamy

Citation:

Citation: Vivekanandan, Subramanian; Ahuja, Shivani; Im, Sang-Choul; Waskell, Lucy; Ramamoorthy, Ayyalusamy. "(1)H, (13)C and (15)N resonance assignments for the full-length mammalian cytochrome b5 in a membrane environment." Biomol. NMR Assignments ., .-. (2013).
PubMed: 24105099

Assembly members:

Assembly members:
Full-length_cytochrome_b5, polymer, 134 residues, 15350 Da.
entity_HEB, non-polymer, 618.503 Da.

Natural source:

Natural source: Common Name: rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Full-length_cytochrome_b5: MAAQSDKDVKYYTLEEIKKH NHSKSTWLILHHKVYDLTKF LEEHPGGEEVLREQAGGDAT ENFEDVGHSTDARELSKTFI IGELHPDDRSKLSKPMETLI TTVDSNSSWWTNWVIPAISA LIVALMYRLYMADD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	157
15N chemical shifts	101
1H chemical shifts	681

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Full-length cytochrome b5	1
2	heme B	2

Entities:

Entity 1, Full-length cytochrome b5 134 residues - 15350 Da.

1

MET

ALA

GLN

SER

ASP

LYS

ASP

VAL

LYS

2

TYR

THR

LEU

GLU

ILE

LYS

HIS

3

ASN

HIS

SER

LYS

SER

THR

TRP

LEU

ILE

LEU

4

HIS

LYS

VAL

TYR

ASP

LEU

THR

LYS

PHE

5

LEU

GLU

HIS

PRO

GLY

GLU

VAL

6

LEU

ARG

GLU

GLN

ALA

GLY

ASP

ALA

THR

7

GLU

ASN

PHE

GLU

ASP

VAL

GLY

HIS

SER

THR

8

ASP

ALA

ARG

GLU

LEU

SER

LYS

THR

PHE

ILE

9

ILE

GLY

GLU

LEU

HIS

PRO

ASP

ARG

SER

10

LYS

LEU

SER

LYS

PRO

MET

GLU

THR

LEU

ILE

11

THR

VAL

ASP

SER

ASN

SER

TRP

12

THR

ASN

TRP

VAL

ILE

PRO

ALA

ILE

SER

ALA

13

LEU

ILE

VAL

ALA

LEU

MET

TYR

ARG

LEU

TYR

14

MET

ALA

ASP

Entity 2, heme B - C34 H34 Fe N4 O4 - 618.503 Da.

1

HEB

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

BMRB	295 4602
PDB	1DO9 2M33
DBJ	BAA01712
GB	AAB03878 AAB32285
PRF	1205244A 1908210A
REF	NP_001164734 NP_001164735
SP	P00169
AlphaFold	P00169

BMRB Entry 18919

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links: