BMRB Entry 16414
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16414
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Title: Interaction of the Second Binding Region Troponin I with the Regulatory Domain of Skeletal Muscle Troponin C as Determined by NMR Spectroscopy PubMed: 9353310
Deposition date: 2009-07-16 Original release date: 2010-03-05
Authors: McKay, Ryan; Tripet, Brian; Hodges, Robert; Sykes, Brian
Citation: McKay, Ryan; Tripet, Brian; Hodges, Robert; Sykes, Brian. "Interaction of the Second Binding Region of Troponin I with the Regulatory Domain of Skeletal Muscle Troponin C as Determined by NMR Spectroscopy" J. Biol. Chem. 272, 28494-28500 (1997).
Assembly members:
TnC, polymer, 90 residues, Formula weight is not available
TnI, polymer, 17 residues, Formula weight is not available
Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: chemical synthesis Host organism: Gallus gallus
Entity Sequences (FASTA):
TnC: ASMTDQQAEARAFLSEEMIA
EFKAAFDMFDADGGGDISTK
ELGTVMRMLGQNPTKCELDA
IICEVDEDGSGTIDFEEFLV
MMVRQMKEDA
TnI: RMSADAMLRALLGSKHK
- binding_constants
| Data type | Count |
| binding constants | 2 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TnC | 1 |
| 2 | TnI | 2 |
Entities:
Entity 1, TnC 90 residues - Formula weight is not available
| 1 | ALA | SER | MET | THR | ASP | GLN | GLN | ALA | GLU | ALA | |
| 2 | ARG | ALA | PHE | LEU | SER | GLU | GLU | MET | ILE | ALA | |
| 3 | GLU | PHE | LYS | ALA | ALA | PHE | ASP | MET | PHE | ASP | |
| 4 | ALA | ASP | GLY | GLY | GLY | ASP | ILE | SER | THR | LYS | |
| 5 | GLU | LEU | GLY | THR | VAL | MET | ARG | MET | LEU | GLY | |
| 6 | GLN | ASN | PRO | THR | LYS | CYS | GLU | LEU | ASP | ALA | |
| 7 | ILE | ILE | CYS | GLU | VAL | ASP | GLU | ASP | GLY | SER | |
| 8 | GLY | THR | ILE | ASP | PHE | GLU | GLU | PHE | LEU | VAL | |
| 9 | MET | MET | VAL | ARG | GLN | MET | LYS | GLU | ASP | ALA |
Entity 2, TnI 17 residues - Formula weight is not available
| 1 | ARG | MET | SER | ALA | ASP | ALA | MET | LEU | ARG | ALA | ||||
| 2 | LEU | LEU | GLY | SER | LYS | HIS | LYS |
Samples:
sample_1: TnC, [U-50% 15N; 2H], 1.36 ± 0.03 mM; imidazole 10 mM; DSS 0.1 mM; potassium chloride 0 mM; calcium chloride 5.4 mM; TnI 1.9 mM; H2O 90%; D2O 10%
sample_2: TnC, [U-50% 15N; 2H], 1.54 ± 0.1 mM; imidazole 10 mM; DSS 0.1 mM; potassium chloride 100 mM; calcium chloride 6 mM; TnI 2.72 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 6.85; pressure: 1 atm; temperature: 304 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
PIPP, Garrett - data analysis
NMR spectrometers:
- Varian Unity 600 MHz