BMRB Entry 16414

Name: Interaction of the Second Binding Region Troponin I with the Regulatory Domain of Skeletal Muscle Troponin C as Determined by NMR Spectroscopy
Published: 2010-03-05

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16414

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Interaction of the Second Binding Region Troponin I with the Regulatory Domain of Skeletal Muscle Troponin C as Determined by NMR Spectroscopy

Deposition date:

2009-07-16

Original release date:

2010-03-05

Authors:

McKay, Ryan; Tripet, Brian; Hodges, Robert; Sykes, Brian

Citation:

Citation: McKay, Ryan; Tripet, Brian; Hodges, Robert; Sykes, Brian. "Interaction of the Second Binding Region of Troponin I with the Regulatory Domain of Skeletal Muscle Troponin C as Determined by NMR Spectroscopy" J. Biol. Chem. 272, 28494-28500 (1997).
PubMed: 9353310

Assembly members:

Assembly members:
TnC, polymer, 90 residues, Formula weight is not available
TnI, polymer, 17 residues, Formula weight is not available

Natural source:

Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: chemical synthesis Host organism: Gallus gallus

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TnC: ASMTDQQAEARAFLSEEMIA EFKAAFDMFDADGGGDISTK ELGTVMRMLGQNPTKCELDA IICEVDEDGSGTIDFEEFLV MMVRQMKEDA
TnI: RMSADAMLRALLGSKHK

Data sets:

binding_constants

Data type	Count
binding constants	2

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TnC	1
2	TnI	2

Entities:

Entity 1, TnC 90 residues - Formula weight is not available

1	ALA	SER	MET	THR	ASP	GLN	GLN	ALA	GLU	ALA
2	ARG	ALA	PHE	LEU	SER	GLU	GLU	MET	ILE	ALA
3	GLU	PHE	LYS	ALA	ALA	PHE	ASP	MET	PHE	ASP
4	ALA	ASP	GLY	GLY	GLY	ASP	ILE	SER	THR	LYS
5	GLU	LEU	GLY	THR	VAL	MET	ARG	MET	LEU	GLY
6	GLN	ASN	PRO	THR	LYS	CYS	GLU	LEU	ASP	ALA
7	ILE	ILE	CYS	GLU	VAL	ASP	GLU	ASP	GLY	SER
8	GLY	THR	ILE	ASP	PHE	GLU	GLU	PHE	LEU	VAL
9	MET	MET	VAL	ARG	GLN	MET	LYS	GLU	ASP	ALA

Entity 2, TnI 17 residues - Formula weight is not available

1

ARG

MET

SER

ALA

ASP

ALA

MET

LEU

ARG

ALA

2

LEU

GLY

SER

LYS

HIS

LYS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1

BMRB	4232 4401 5738
PDB	1AVS 1BLQ 1NPQ 1SKT 1TNP 1TNQ 1TRF 1ZAC 1NPQ 1YTZ 1YV0 2W49 2W4U
REF	XP_005057071 XP_009321443 XP_009582608 XP_009992324 XP_013053940 NP_001011110 NP_001079556 NP_990748 XP_001518819 XP_002199061
EMBL	CAA27447
GB	AAA61952 AAB00122 AAH44282 AAH84508 AAL86905
SP	P68246 P68247
AlphaFold	P68246 P68247

BMRB Entry 16414

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	ALA	SER	MET	THR	ASP	GLN	GLN	ALA	GLU	ALA
2	ARG	ALA	PHE	LEU	SER	GLU	GLU	MET	ILE	ALA
3	GLU	PHE	LYS	ALA	ALA	PHE	ASP	MET	PHE	ASP
4	ALA	ASP	GLY	GLY	GLY	ASP	ILE	SER	THR	LYS
5	GLU	LEU	GLY	THR	VAL	MET	ARG	MET	LEU	GLY
6	GLN	ASN	PRO	THR	LYS	CYS	GLU	LEU	ASP	ALA
7	ILE	ILE	CYS	GLU	VAL	ASP	GLU	ASP	GLY	SER
8	GLY	THR	ILE	ASP	PHE	GLU	GLU	PHE	LEU	VAL
9	MET	MET	VAL	ARG	GLN	MET	LYS	GLU	ASP	ALA

1	ALA	SER	MET	THR	ASP	GLN	GLN	ALA	GLU	ALA
2	ARG	ALA	PHE	LEU	SER	GLU	GLU	MET	ILE	ALA
3	GLU	PHE	LYS	ALA	ALA	PHE	ASP	MET	PHE	ASP
4	ALA	ASP	GLY	GLY	GLY	ASP	ILE	SER	THR	LYS
5	GLU	LEU	GLY	THR	VAL	MET	ARG	MET	LEU	GLY
6	GLN	ASN	PRO	THR	LYS	CYS	GLU	LEU	ASP	ALA
7	ILE	ILE	CYS	GLU	VAL	ASP	GLU	ASP	GLY	SER
8	GLY	THR	ILE	ASP	PHE	GLU	GLU	PHE	LEU	VAL
9	MET	MET	VAL	ARG	GLN	MET	LYS	GLU	ASP	ALA

1	ALA	SER	MET	THR	ASP	GLN	GLN	ALA	GLU	ALA
2	ARG	ALA	PHE	LEU	SER	GLU	GLU	MET	ILE	ALA
3	GLU	PHE	LYS	ALA	ALA	PHE	ASP	MET	PHE	ASP
4	ALA	ASP	GLY	GLY	GLY	ASP	ILE	SER	THR	LYS
5	GLU	LEU	GLY	THR	VAL	MET	ARG	MET	LEU	GLY
6	GLN	ASN	PRO	THR	LYS	CYS	GLU	LEU	ASP	ALA
7	ILE	ILE	CYS	GLU	VAL	ASP	GLU	ASP	GLY	SER
8	GLY	THR	ILE	ASP	PHE	GLU	GLU	PHE	LEU	VAL
9	MET	MET	VAL	ARG	GLN	MET	LYS	GLU	ASP	ALA