BMRB Entry 4232
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4232
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Title: N-Domain of Troponin C from Chicken Skeletal Muscle.
Deposition date: 1998-09-27 Original release date: 1999-05-25
Authors: Tsuda, Sakae; Miura, A.; Gagne, S.; Spyracopoulos, L.; Sykes, Brian
Citation: Tsuda, Sakae; Miura, A.; Gagne, S.; Spyracopoulos, L.; Sykes, Brian. "Low Temperature-Induced Structural Changes in the Apo Regulatory Domain of Skeletal Muscle Troponin C" Biochemistry 38, 5693-5700 (1999).
Assembly members:
apoTroponin-C, polymer, 90 residues, Formula weight is not available
Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a
Entity Sequences (FASTA):
apoTroponin-C: ASMTDQQAEARAFLSEEMIA
EFKAAFDMFDADGGGDISTK
ELGTVMRMLGQNPTKEELDA
IIEEVDEDGSGTIDFEEFLV
MMVRQMKEDA
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 259 |
| 15N chemical shifts | 184 |
| 1H chemical shifts | 770 |
| coupling constants | 81 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | apoNTnC | 1 |
Entities:
Entity 1, apoNTnC 90 residues - Formula weight is not available
| 1 | ALA | SER | MET | THR | ASP | GLN | GLN | ALA | GLU | ALA | |
| 2 | ARG | ALA | PHE | LEU | SER | GLU | GLU | MET | ILE | ALA | |
| 3 | GLU | PHE | LYS | ALA | ALA | PHE | ASP | MET | PHE | ASP | |
| 4 | ALA | ASP | GLY | GLY | GLY | ASP | ILE | SER | THR | LYS | |
| 5 | GLU | LEU | GLY | THR | VAL | MET | ARG | MET | LEU | GLY | |
| 6 | GLN | ASN | PRO | THR | LYS | GLU | GLU | LEU | ASP | ALA | |
| 7 | ILE | ILE | GLU | GLU | VAL | ASP | GLU | ASP | GLY | SER | |
| 8 | GLY | THR | ILE | ASP | PHE | GLU | GLU | PHE | LEU | VAL | |
| 9 | MET | MET | VAL | ARG | GLN | MET | LYS | GLU | ASP | ALA |
Samples:
sample_one: apoTroponin-C, [U-13C; U-15N], 1.5 mM; KCl 50 mM
sample_conditions_one: pH: 6.7; pressure: 1 atm; temperature: 277 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| CBCACONNH | sample_one | not available | sample_conditions_one |
| 15N-NOESY | sample_one | not available | sample_conditions_one |
| HNHA | sample_one | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
- Varian UNITY 500 MHz
- Varian UNITY 600 MHz
Related Database Links:
| BMRB | 16414 4401 |
| PDB | |
| GB | EMP28292 KFU86923 |
| REF | XP_007067817 XP_009321443 XP_009582608 XP_009693659 XP_009992324 |
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