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BMRB Entry 16497

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16497
MolProbity Validation Chart

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Title: 1H, 13C, and 15N Chemical Shift Assignments for the Integrin AlphaIIb-Beta3 Transmembrane Complex   PubMed: 19279667

Deposition date: 2009-09-15 Original release date: 2009-09-24

Authors: Lau, Tong-Lay; Ulmer, Tobias

Citation: Lau, Tong-Lay; Kim, Chungho; Ginsberg, Mark; Ulmer, Tobias. "The structure of the integrin alphaIIbbeta3 transmembrane complex explains integrin transmembrane signalling"  EMBO J. 28, 1351-1361 (2009).

Assembly members:
Integrin_aIIb_transmembrane_segment,_b3_complexed, polymer, 42 residues, Formula weight is not available
Integrin_b3_transmembrane_segment,_aIIb_complexed, polymer, 43 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-44

Entity Sequences (FASTA):
Integrin_aIIb_transmembrane_segment,_b3_complexed: GALEERAIPIWWVLVGVLGG LLLLTILVLAMWKVGFFKRN RP
Integrin_b3_transmembrane_segment,_aIIb_complexed: PESPKGPDILVVLLSVMGAI LLIGLAALLIWKLLITIHDR KEF

Data sets:
Data typeCount
13C chemical shifts203
15N chemical shifts71
1H chemical shifts462

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Integrin aIIb transmembrane segment1
2Integrin b3 transmembrane segment2

Entities:

Entity 1, Integrin aIIb transmembrane segment 42 residues - Formula weight is not available

1   GLYALALEUGLUGLUARGALAILEPROILE
2   TRPTRPVALLEUVALGLYVALLEUGLYGLY
3   LEULEULEULEUTHRILELEUVALLEUALA
4   METTRPLYSVALGLYPHEPHELYSARGASN
5   ARGPRO

Entity 2, Integrin b3 transmembrane segment 43 residues - Formula weight is not available

1   PROGLUSERPROLYSGLYPROASPILELEU
2   VALVALLEULEUSERVALMETGLYALAILE
3   LEULEUILEGLYLEUALAALALEULEUILE
4   TRPLYSLEULEUILETHRILEHISASPARG
5   LYSGLUPHE

Samples:

sample_1: Integrin aIIb transmembrane segment, b3 complexed, [U-100% 13C; U-100% 15N; U-80% 2H], 0.4 mM; Integrin b3 transmembrane segment, aIIb complexed 0.8 mM; HEPES/NaOH 25 mM; H2O 95%; D2O 5%

sample_2: Integrin aIIb transmembrane segment, b3 complexed 0.9 mM; Integrin b3 transmembrane segment, aIIb complexed, [U-100% 13C; U-100% 15N; U-80% 2H], 0.6 mM; HEPES/NaOH 25 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.025 M; pH: 7.4; pressure: 1 atm; temperature: 301.2 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 16496 16496 17185
PDB
DBJ BAG37735 BAI46167 BAG62151 BAJ17755
EMBL CAA29987
GB AAA35926 AAA52597 AAA53150 AAA60114 AAA65936 AAA35927 AAA36121 AAA52589 AAA52600 AAA60122
PRF 1403305A
REF NP_000410 XP_001114526 XP_001150497 XP_002827479 XP_003282434 NP_000203 XP_002748172 XP_002834363 XP_003270746 XP_004041501
SP P08514 P53711 P05106
AlphaFold P53711 P08514 P05106

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