BMRB Entry 16512
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16512
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Title: NMR chemical shift of synaptobrevin fragment 60-116 PubMed: 19918058
Deposition date: 2009-09-23 Original release date: 2009-10-05
Authors: Ellena, Jeffrey; Liang, Binyong; Wiktor, Maciej; Stein, Alexander; Cafiso, David; Jahn, Reinhard; Tamm, Lukas
Citation: Ellena, Jeffrey; Liang, Binyong; Wiktor, Maciej; Stein, Alexander; Cafiso, David; Jahn, Reinhard; Tamm, Lukas. "Dynamic structure of lipid-bound synaptobrevin suggests a nucleation-propagation mechanism for trans-SNARE complex formation." Proc. Natl. Acad. Sci. U.S.A. 106, 20306-20311 (2009).
Assembly members:
syb60-116, polymer, 57 residues, Formula weight is not available
Natural source: Common Name: Norway Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
syb60-116: LSELDDRADALQAGASQFET
SAAKLKRKYWWKNLKMMIIL
GVICAIILIIIIVYFST
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 151 |
| 15N chemical shifts | 55 |
| 1H chemical shifts | 200 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | syb60-116 | 1 |
Entities:
Entity 1, syb60-116 57 residues - Formula weight is not available
| 1 | LEU | SER | GLU | LEU | ASP | ASP | ARG | ALA | ASP | ALA | ||||
| 2 | LEU | GLN | ALA | GLY | ALA | SER | GLN | PHE | GLU | THR | ||||
| 3 | SER | ALA | ALA | LYS | LEU | LYS | ARG | LYS | TYR | TRP | ||||
| 4 | TRP | LYS | ASN | LEU | LYS | MET | MET | ILE | ILE | LEU | ||||
| 5 | GLY | VAL | ILE | CYS | ALA | ILE | ILE | LEU | ILE | ILE | ||||
| 6 | ILE | ILE | VAL | TYR | PHE | SER | THR |
Samples:
sample_1: syb60-116, [U-99% 13C; U-99% 15N], 1 mM; DPC 200 mM; MES 20 mM; NaCl 150 mM; DTT 5 mM; EDTA 1 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 150 mM; pH: 6; pressure: 1 atm; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 16508 16514 4272 |
| PDB | |
| DBJ | BAC34586 BAC34723 BAC41125 BAE38479 BAE40328 |
| EMBL | CAA12385 CAA53792 CAJ81663 |
| GB | AAA42321 AAA60604 AAB03463 AAB03490 AAB27554 |
| PRF | 1918358A |
| REF | NP_001016972 NP_001027992 NP_001034578 NP_001039380 NP_001088233 |
| SP | P63024 P63025 P63026 P63027 P63044 |
| TPG | DAA18807 DAA21271 |
| AlphaFold | P63044 P63025 P63024 P63026 P63027 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts