BMRB Entry 16663
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16663
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Title: NMR STRUCTURE OF HUMAN INSULIN MUTANT GLY-B8-D-ALA, HIS-B10-2 ASP PRO-B28-LYS, LYS-B29-PRO, 20 STRUCTURES PubMed: 21204007
Deposition date: 2010-01-04 Original release date: 2012-08-03
Authors: Hua, Q.; Wan, Z.; Zhao, M.; Jia, W.; Huang, K.; Weiss, M.
Citation: Hua, Qingxin. "Insulin: a small protein with a long journey." Protein Cell 1, 537-551 (2010).
Assembly members:
entity, chain 1, polymer, 21 residues, 2383.700 Da.
entity, chain 2, polymer, 30 residues, 3424.948 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pBR322
Entity Sequences (FASTA):
entity, chain 1: GIVEQCCTSICSLYQLENYC
N
entity, chain 2: FVNQHLCXSDLVEALYLVCG
ERGFFYTKPT
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 343 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | entity, chain 1 | 1 |
2 | entity, chain 2 | 2 |
Entities:
Entity 1, entity, chain 1 21 residues - 2383.700 Da.
1 | GLY | ILE | VAL | GLU | GLN | CYS | CYS | THR | SER | ILE | ||||
2 | CYS | SER | LEU | TYR | GLN | LEU | GLU | ASN | TYR | CYS | ||||
3 | ASN |
Entity 2, entity, chain 2 30 residues - 3424.948 Da.
1 | PHE | VAL | ASN | GLN | HIS | LEU | CYS | DAL | SER | ASP | |
2 | LEU | VAL | GLU | ALA | LEU | TYR | LEU | VAL | CYS | GLY | |
3 | GLU | ARG | GLY | PHE | PHE | TYR | THR | LYS | PRO | THR |
Samples:
sample_1: entity_10.5 0.8 mM; entity_20.5 0.8 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D TOCSY | sample_1 | isotropic | sample_conditions_1 |
NOESY | sample_1 | isotropic | sample_conditions_1 |
COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMR v3.5, Varian - structure solution
NMR spectrometers:
- BRUKER DRX 700 MHz