BMRB Entry 17462
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17462
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Title: EGF-like Module Pair 3-4 in Vitamin K-dependent Protein S: Modulation of Calcium Affinity of Module 4 by Module 3, and Interaction with Factor X
Deposition date: 2011-02-11 Original release date: 2012-06-06
Authors: Stenberg, Yvonne; Muranyi, Andreas; Steen, Christina; Thulin, Eva; Drakenberg, Torbjorn; Stenflo, Johan
Citation: Stenberg, Yvonne; Muranyi, Andreas; Steen, Christina; Thulin, Eva; Drakenberg, Torbjorn; Stenflo, Johan. "EGF-like Module Pair 3-4 in Vitamin K-dependent Protein S: Modulation of Calcium Affinity of Module 4 by Module 3, and Interaction with Factor X" J. Mol. Biol. 293, 653-665 (1999).
Assembly members:
EGF, polymer, 109 residues, 40.078 Da.
CA, non-polymer, 40.078 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16b
Entity Sequences (FASTA):
EGF: MGHHHHHHHHHHSSGHIEGR
HMKDVDECSLKPSICGTAVC
KNIPGDFECECPEGYRYNLK
SKSCEDIDECSENMCAQLCV
NYPGGYTCYCDGKKGFKLAQ
DQKSCEVVS
- binding_constants
- pKa_values
| Data type | Count |
| binding constants | 2 |
| pKa values | 1 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EGF | 1 |
| 2 | CA | 2 |
Entities:
Entity 1, EGF 109 residues - 40.078 Da.
| 1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | HIS | HIS | SER | SER | GLY | HIS | ILE | GLU | GLY | ARG | ||||
| 3 | HIS | MET | LYS | ASP | VAL | ASP | GLU | CYS | SER | LEU | ||||
| 4 | LYS | PRO | SER | ILE | CYS | GLY | THR | ALA | VAL | CYS | ||||
| 5 | LYS | ASN | ILE | PRO | GLY | ASP | PHE | GLU | CYS | GLU | ||||
| 6 | CYS | PRO | GLU | GLY | TYR | ARG | TYR | ASN | LEU | LYS | ||||
| 7 | SER | LYS | SER | CYS | GLU | ASP | ILE | ASP | GLU | CYS | ||||
| 8 | SER | GLU | ASN | MET | CYS | ALA | GLN | LEU | CYS | VAL | ||||
| 9 | ASN | TYR | PRO | GLY | GLY | TYR | THR | CYS | TYR | CYS | ||||
| 10 | ASP | GLY | LYS | LYS | GLY | PHE | LYS | LEU | ALA | GLN | ||||
| 11 | ASP | GLN | LYS | SER | CYS | GLU | VAL | VAL | SER |
Entity 2, CA - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: EGF, [U-15N], 0.214 mM; sodium chloride 150 mM; sodium chloride 0.1 mM; DSS 1 mM; calcium chloride0.040 – 0.200 M; H2O 90%; D2O 10%
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
FELIX v97, Accelrys Software Inc. - processing
NMR spectrometers:
- Varian INOVA 600 MHz