BMRB Entry 4729
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4729
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Title: 1H, 15N, 13CA, 13CB, 13C' assignments of the EGF-like module pair 3-4 from vitamin K-dependent protein S
Deposition date: 2000-04-28 Original release date: 2000-05-11
Authors: Muranyi, Andreas; Evenas, Johan; Stenberg, Yvonne; Stenflo, Johan; Drakenberg, Torbjorn
Citation: Muranyi, Andreas; Evenas, Johan; Stenberg, Yvonne; Stenflo, Johan; Drakenberg, Torbjorn. "1H, 15N and 13C assignments and secondary structure of the EGF-like module pair 3-4 from vitamin K-dependent protein S" FEBS Lett. ., .-..
Assembly members:
EGF-like module pair 3-4 from human vitamin K-dependent protein S, polymer, 89 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
EGF-like module pair 3-4 from human vitamin K-dependent protein S: HMKDVDECSLKPSICGTAVC
KNIPGDFECECPEGYRYNLK
SKSCEDIDECSENMCAQLCV
NYPGGYTCYCDGKKGFKLAQ
DQKSCEVVS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 947 |
| 13C chemical shifts | 404 |
| 15N chemical shifts | 170 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | monomer 1 | 1 |
| 2 | monomer 2 | 1 |
| 3 | Ca 1 | 2 |
| 4 | Ca 2 | 2 |
Entities:
Entity 1, monomer 1 89 residues - Formula weight is not available
| 1 | HIS | MET | LYS | ASP | VAL | ASP | GLU | CYS | SER | LEU | ||||
| 2 | LYS | PRO | SER | ILE | CYS | GLY | THR | ALA | VAL | CYS | ||||
| 3 | LYS | ASN | ILE | PRO | GLY | ASP | PHE | GLU | CYS | GLU | ||||
| 4 | CYS | PRO | GLU | GLY | TYR | ARG | TYR | ASN | LEU | LYS | ||||
| 5 | SER | LYS | SER | CYS | GLU | ASP | ILE | ASP | GLU | CYS | ||||
| 6 | SER | GLU | ASN | MET | CYS | ALA | GLN | LEU | CYS | VAL | ||||
| 7 | ASN | TYR | PRO | GLY | GLY | TYR | THR | CYS | TYR | CYS | ||||
| 8 | ASP | GLY | LYS | LYS | GLY | PHE | LYS | LEU | ALA | GLN | ||||
| 9 | ASP | GLN | LYS | SER | CYS | GLU | VAL | VAL | SER |
Entity 2, Ca 1 - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: EGF-like module pair 3-4 from human vitamin K-dependent protein S 1.5 mM
sample_2: EGF-like module pair 3-4 from human vitamin K-dependent protein S, [U-99% 15N], 0.5 – 1.5 mM
sample_3: EGF-like module pair 3-4 from human vitamin K-dependent protein S, [U-99% 15N; U-99% 13C], 1.2 – 1.9 mM
Ex-cond_1: pH: 6.0; temperature: 309 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H NOESY, with mixing times 80 and 150 ms. | not available | not available | Ex-cond_1 |
| 1H TOCSY, with DIPSI isotropic mixing times of 40 and 110 ms. | not available | not available | Ex-cond_1 |
| 1H-15N HSQC | not available | not available | Ex-cond_1 |
| 1H-15N TOCSY-HSQC, with a DIPSI mixing sequence (40-110 ms) | not available | not available | Ex-cond_1 |
| 1H-15N NOESY-HSQC, with mixing times of 80 and 150 ms | not available | not available | Ex-cond_1 |
| HNCA | not available | not available | Ex-cond_1 |
| HN(CO)CA | not available | not available | Ex-cond_1 |
| HNCACB | not available | not available | Ex-cond_1 |
| HNCO | not available | not available | Ex-cond_1 |
Software:
FELIX v98.0 -
XEASY v1.3.13 -
NMR spectrometers:
- Varain Unity-Inova 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts