Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18305
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Citation: Moorman, Veronica; Valentine, Kathleen; Wand, A. Joshua. "The dynamical response of hen egg white lysozyme to the binding of a carbohydrate ligand." Protein Sci. 21, 1066-1073 (2012).
PubMed: 22593013
Assembly members:
HEWL, polymer, 129 residues, Formula weight is not available
N-ACETYL-D-GLUCOSAMINE, non-polymer, 221.208 Da.
Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a
Data type | Count |
order parameters | 138 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | HEWL | 1 |
2 | chitotriose | 2 |
Entity 1, HEWL 129 residues - Formula weight is not available
1 | LYS | VAL | PHE | GLY | ARG | CYS | GLU | LEU | ALA | ALA | ||||
2 | ALA | MET | LYS | ARG | HIS | GLY | LEU | ASP | ASN | TYR | ||||
3 | ARG | GLY | TYR | SER | LEU | GLY | ASN | TRP | VAL | CYS | ||||
4 | ALA | ALA | LYS | PHE | GLU | SER | ASN | PHE | ASN | THR | ||||
5 | GLN | ALA | THR | ASN | ARG | ASN | THR | ASP | GLY | SER | ||||
6 | THR | ASP | TYR | GLY | ILE | LEU | GLN | ILE | ASN | SER | ||||
7 | ARG | TRP | TRP | CYS | ASN | ASP | GLY | ARG | THR | PRO | ||||
8 | GLY | SER | ARG | ASN | LEU | CYS | ASN | ILE | PRO | CYS | ||||
9 | SER | ALA | LEU | LEU | SER | SER | ASP | ILE | THR | ALA | ||||
10 | SER | VAL | ASN | CYS | ALA | LYS | LYS | ILE | VAL | SER | ||||
11 | ASP | GLY | ASN | GLY | MET | ASN | ALA | TRP | VAL | ALA | ||||
12 | TRP | ARG | ASN | ARG | CYS | LYS | GLY | THR | ASP | VAL | ||||
13 | GLN | ALA | TRP | ILE | ARG | GLY | CYS | ARG | LEU |
Entity 2, chitotriose - C8 H15 N O6 - 221.208 Da.
1 | NAG |
sample_1: HEWL, [U-100% 13C; U-100% 15N; U-55% 2H], 410 uM; chitotriose 492 uM; acetate 100 mM; sodium azide 0.04%; H2O 90%; D2O 10%
sample_conditions_1: temperature: 308.15 K; pH: 4.7; pressure: 1 atm
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
N T1 relaxation | sample_1 | isotropic | sample_conditions_1 |
N T1 relaxation | sample_1 | isotropic | sample_conditions_1 |
N T2 relaxation | sample_1 | isotropic | sample_conditions_1 |
N T2 relaxation | sample_1 | isotropic | sample_conditions_1 |
N NOE relaxation | sample_1 | isotropic | sample_conditions_1 |
N NOE relaxation | sample_1 | isotropic | sample_conditions_1 |
IzCz relaxation | sample_1 | isotropic | sample_conditions_1 |
IzCz relaxation | sample_1 | isotropic | sample_conditions_1 |
IzCzDz relaxation | sample_1 | isotropic | sample_conditions_1 |
IzCzDz relaxation | sample_1 | isotropic | sample_conditions_1 |
IzCzDy relaxation | sample_1 | isotropic | sample_conditions_1 |
IzCzDy relaxation | sample_1 | isotropic | sample_conditions_1 |
TOPSPIN, Bruker Biospin - collection
Felix, Accelrys Software Inc. - processing
in-house_software, wandlab.org - data analysis
BMRB | 1093 1104 1105 11052 1650 1651 1653 1770 1772 1798 1799 1800 1801 1802 1803 18304 1865 1881 1882 1883 1884 1975 1977 2231 2446 2786 2858 2917 2957 4562 |
PDB | |
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AlphaFold | P00698 P00699 P00700 Q7LZP9 Q7LZQ0 |