BMRB Entry 30797

Name: Dengue 5'UTR SLA
Published: 2021-09-22

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PDB ID: 7k4l
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30797
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Dengue 5'UTR SLA

Deposition date: 2020-09-15 Original release date: 2021-09-22

Authors: Sun, Y.

Citation: Sun, Y.. "Solution NMR Structure of Denv1 5'UTR SLA region" .

Assembly members:
entity_1, polymer, 36 residues, 11521.802 Da.

Natural source: Common Name: Escherichia phage T7 Taxonomy ID: 10760 Superkingdom: Viruses Kingdom: not available Genus/species: Teseptimavirus Escherichia virus T7

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: GGAGUGGUUAGUCUACGUUU CGACGUAGUUCUAACC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	126

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 36 residues - 11521.802 Da.

1

G

A

G

U

G

U

A

2

G

U

C

U

A

C

G

U

3

C

G

A

C

G

U

A

G

U

4

C

U

A

C

Samples:

sample_1: DenvSLA 200 uM; potassium phosphate 10 mM; potassium chloride 10 mM

sample_2: DenvSLA, [U-100% 2H], 200 uM; potassium phosphate 10 mM; potassium chloride 10 mM

sample_conditions_1: ionic strength: 40 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1

Software:

TopSpin, Bruker Biospin - processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMRFAM-SPARKY, Lee W, Tonelli M, Markley JL - chemical shift assignment

NMR spectrometers:

Bruker AVANCE II 600 MHz
Bruker AVANCE II 500 MHz