BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34674

Title: Solution structure of an intramolecular RNA G-quadruplex formed by the 6A8U17U mutant from a 22mer guanine-rich sequence within the 5'UTR of BCL-2 proto onco-gene

Deposition date: 2021-10-29 Original release date: 2022-11-08

Authors: Wang, Z.; Jurt, S.; Dominguez-Martin, A.; Johannsen, S.; Sigel, R.

Citation: Wang, Z.; Jurt, S.; Dominguez-Martin, A.; Johannsen, S.; Sigel, R.. "Solution structure of an intramolecular RNA G-quadruplex formed by the 6A8U17U mutant from a 22mer guanine-rich sequence within the 5'UTR of BCL-2 proto onco-gene"  .

Assembly members:
entity_1, polymer, 22 residues, 7202.299 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: GGGCCAUUGGGUGGGAUCUG GG

Data sets:
Data typeCount
13C chemical shifts179
15N chemical shifts69
1H chemical shifts184

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 22 residues - 7202.299 Da.

1   GGGCCAUUGG
2   GUGGGAUCUG
3   GG

Samples:

sample_1: BCL-2 6A8U17U 1.0 mM

sample_2: BCL-2 6A8U17U 1.0 mM

sample_3: BCL-2 6A8U17U, [G & U 15N-labeled], 0.5 mM

sample_4: BCL-2 6A8U17U, [G & U 15N-labeled], 0.5 mM

sample_5: BCL-2 6A8U17U, [A & C 15N-labeled], 0.5 mM

sample_6: BCL-2 6A8U17U, [A 13C, 15N-labeled], 0.5 mM

sample_7: BCL-2 6A8U17U, [C 13C, 15N-labeled], 0.3 mM

sample_8: BCL-2 6A8U17U, [G 13C-labeled], 0.3 mM

sample_9: BCL-2 6A8U17U, [U 13C, 15N-labeled], 0.5 mM

sample_conditions_1: ionic strength: 2 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 2 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

sample_conditions_3: ionic strength: 2 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

sample_conditions_4: ionic strength: 2 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

sample_conditions_5: ionic strength: 2 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

sample_conditions_6: ionic strength: 2 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

sample_conditions_7: ionic strength: 2 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

sample_conditions_8: ionic strength: 2 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

sample_conditions_9: ionic strength: 2 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2
2D 1H-1H TOCSYsample_2isotropicsample_conditions_2
2D 1H-13C HSQCsample_2isotropicsample_conditions_2
2D 1H-15N HSQCsample_3isotropicsample_conditions_3
JR-HMBCsample_1isotropicsample_conditions_1
H8N2 HNN-COSYsample_3isotropicsample_conditions_3
H1N2 HNN-COSYsample_3isotropicsample_conditions_3
H1pC6/8 HMBCsample_2isotropicsample_conditions_2
long-range 1H-15N HSQCsample_4isotropicsample_conditions_4
long-range 1H-15N HSQCsample_5isotropicsample_conditions_5
constant-time 1H-13C HSQCsample_6isotropicsample_conditions_6
constant-time 1H-13C HSQCsample_7isotropicsample_conditions_7
constant-time 1H-13C HSQCsample_8isotropicsample_conditions_8
constant-time 1H-13C HSQCsample_9isotropicsample_conditions_9

Software:

TopSpin v4.0.6, Bruker Biospin - collection

NMRFAM-SPARKY v1.414, Lee W, Tonelli M, Markley JL - chemical shift assignment, peak picking

X-PLOR NIH v3.0, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Bruker AVANCE 700 MHz