BMRB Entry 4069
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4069
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Title: Secondary Structure Determination of 15N-Labelled Human Long-[Arg-3]-Insulin-Like Growth Factor-I by Multidimensional NMR Spectroscopy
Deposition date: 1997-10-28 Original release date: 2002-08-12
Authors: Laajoki, Leanne; Le Breton, Eugene; Shooter, Gary; Wallace, John; Francis, Geoff; Carver, John; Keniry, Max
Citation: Laajoki, Leanne; Le Breton, Eugene; Shooter, Gary; Wallace, John; Francis, Geoff; Carver, John; Keniry, Max. "Secondary Structure Determination of 15N-Labelled Human Long-[Arg-3]-Insulin-Like Growth Factor-I by Multidimensional NMR Spectroscopy" FEBS Lett. ., .-. (1997).
Assembly members:
Long-[Arg-3]-Insulin-Like Growth Factor-I, polymer, 83 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: p[Met1]-pGH(11)-Val-Asn-IGF-I
Entity Sequences (FASTA):
Long-[Arg-3]-Insulin-Like Growth Factor-I: MFPAMPLSSLFVNGPRTLCG
AELVDALQFVCGDRGFYFNK
PTGYGSSSRRAPQTGIVDEC
CFRSCDLRRLEMYCAPLKPA
KSA
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 76 |
| 1H chemical shifts | 549 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Long-[Arg-3]-IGF-I | 1 |
Entities:
Entity 1, Long-[Arg-3]-IGF-I 83 residues - Formula weight is not available
| 1 | MET | PHE | PRO | ALA | MET | PRO | LEU | SER | SER | LEU | ||||
| 2 | PHE | VAL | ASN | GLY | PRO | ARG | THR | LEU | CYS | GLY | ||||
| 3 | ALA | GLU | LEU | VAL | ASP | ALA | LEU | GLN | PHE | VAL | ||||
| 4 | CYS | GLY | ASP | ARG | GLY | PHE | TYR | PHE | ASN | LYS | ||||
| 5 | PRO | THR | GLY | TYR | GLY | SER | SER | SER | ARG | ARG | ||||
| 6 | ALA | PRO | GLN | THR | GLY | ILE | VAL | ASP | GLU | CYS | ||||
| 7 | CYS | PHE | ARG | SER | CYS | ASP | LEU | ARG | ARG | LEU | ||||
| 8 | GLU | MET | TYR | CYS | ALA | PRO | LEU | LYS | PRO | ALA | ||||
| 9 | LYS | SER | ALA |
Samples:
sample_one: Long-[Arg-3]-Insulin-Like Growth Factor-I, [U-15N], 1.0 mM; H2O 90%; D2O 10%
sample_two: Long-[Arg-3]-Insulin-Like Growth Factor-I 1.3 mM; D2O 100%
sample_conditions_IGF-I: pH: 4.1; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Varian INOVA-500 500 MHz
- Varian INOVA-600 600 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts