BMRB Entry 4278
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4278
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Title: Backbone 1H and 15N Chemical Shift Assignments for Long-[L60]-IGF-I, an Insulin-Like Growth Factor 1 Analogue (IGF-I)
Deposition date: 1998-12-07 Original release date: 2002-07-12
Authors: Laajoki, Leanne; Milner, Steve; Francis, Geoff; Carver, John; Keniry, Max
Citation: Laajoki, Leanne; Milner, Steve; Francis, Geoff; Carver, John; Keniry, Max. "1H and 15N Assignments of Long-[L60]-IGF-I, an Insulin-Like Growth Factor I Analogue" J. Biomol. NMR ., .-. (1999).
Assembly members:
IGF-I, polymer, 83 residues, 9139 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: p[Met1]-pGH(11)-Val-Asn-IGF-I
Entity Sequences (FASTA):
IGF-I: MFPAMPLSSLFVNGPETLCG
AELVDALQFVCGDRGFYFNK
PTGYGSSSRRAPQTGIVDEC
CFRSCDLRRLEMLCAPLKPA
KSA
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 531 |
| coupling constants | 59 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Long [L60] IGF I | 1 |
Entities:
Entity 1, Long [L60] IGF I 83 residues - 9139 Da.
| 1 | MET | PHE | PRO | ALA | MET | PRO | LEU | SER | SER | LEU | ||||
| 2 | PHE | VAL | ASN | GLY | PRO | GLU | THR | LEU | CYS | GLY | ||||
| 3 | ALA | GLU | LEU | VAL | ASP | ALA | LEU | GLN | PHE | VAL | ||||
| 4 | CYS | GLY | ASP | ARG | GLY | PHE | TYR | PHE | ASN | LYS | ||||
| 5 | PRO | THR | GLY | TYR | GLY | SER | SER | SER | ARG | ARG | ||||
| 6 | ALA | PRO | GLN | THR | GLY | ILE | VAL | ASP | GLU | CYS | ||||
| 7 | CYS | PHE | ARG | SER | CYS | ASP | LEU | ARG | ARG | LEU | ||||
| 8 | GLU | MET | LEU | CYS | ALA | PRO | LEU | LYS | PRO | ALA | ||||
| 9 | LYS | SER | ALA |
Samples:
sample_one: IGF-I, [U-98% 15N], 1.0 mM
sample_conditions_one: pH: 3.0; temperature: 303 K
sample_conditions_two: pH: 3.0; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_one | not available | not available |
| 2D 1H-15N HSQC-TOCSY | sample_one | not available | not available |
| 2D 1H-15N HSQC-NOESY | sample_one | not available | not available |
| 3D 15N NOESY-HSQC | sample_one | not available | not available |
| 3D 15N TOCSY-HSQC | sample_one | not available | not available |
| 2D 1H NOESY | sample_one | not available | not available |
| 3D 15N HNHA | sample_one | not available | not available |
Software:
VNMR v6.1 - Spectral Processing
XEASY v1.3.10 - Spectral Analysis and Peak Picking
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts