BMRB Entry 4149
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4149
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Title: Solution Structure of Gallium-Substituted Putidaredoxin Mutant: GaPdx C85S
Deposition date: 1998-06-08 Original release date: 1999-02-03
Authors: Pochapsky, Thomas; Kuti, Miklos; Kazanis, Sophia
Citation: Pochapsky, Thomas; Kuti, Miklos; Kazanis, Sophia. "Solution Structure of Gallium-Substituted Putidaredoxin Mutant: GaPdx C85S" J. Biomol. NMR 12, 407-415 (1998).
Assembly members:
putidaredoxin, polymer, 106 residues, Formula weight is not available
GALLIUM (III) ION, non-polymer, 69.723 Da.
Natural source: Common Name: Pseudomonas putida Taxonomy ID: 303 Superkingdom: Eubacteria Kingdom: not available Genus/species: Pseudomonas putida
Experimental source: Production method: recombinant_technology
Entity Sequences (FASTA):
putidaredoxin: SKVVYVSHDGTRRELDVADG
VSLMQAAVSNGIYDIVGDCG
GSASCATCHVYVNEAFTDKV
PAANEREIGMLECVTAELKP
NSRLSCQIIMTPELDGIVVD
VPDRQW
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 446 |
| 15N chemical shifts | 114 |
| 1H chemical shifts | 720 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Pdx-protein | 1 |
| 2 | entity_GA | 2 |
Entities:
Entity 1, Pdx-protein 106 residues - Formula weight is not available
| 1 | SER | LYS | VAL | VAL | TYR | VAL | SER | HIS | ASP | GLY | ||||
| 2 | THR | ARG | ARG | GLU | LEU | ASP | VAL | ALA | ASP | GLY | ||||
| 3 | VAL | SER | LEU | MET | GLN | ALA | ALA | VAL | SER | ASN | ||||
| 4 | GLY | ILE | TYR | ASP | ILE | VAL | GLY | ASP | CYS | GLY | ||||
| 5 | GLY | SER | ALA | SER | CYS | ALA | THR | CYS | HIS | VAL | ||||
| 6 | TYR | VAL | ASN | GLU | ALA | PHE | THR | ASP | LYS | VAL | ||||
| 7 | PRO | ALA | ALA | ASN | GLU | ARG | GLU | ILE | GLY | MET | ||||
| 8 | LEU | GLU | CYS | VAL | THR | ALA | GLU | LEU | LYS | PRO | ||||
| 9 | ASN | SER | ARG | LEU | SER | CYS | GLN | ILE | ILE | MET | ||||
| 10 | THR | PRO | GLU | LEU | ASP | GLY | ILE | VAL | VAL | ASP | ||||
| 11 | VAL | PRO | ASP | ARG | GLN | TRP |
Entity 2, entity_GA - Ga - 69.723 Da.
| 1 | GA |
Samples:
sample_one: gallium putidaredoxin 2 mM; Tris buffer 0.01 M; mercapatoethanol trace
sample_conditions_one: pH: 7.4; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | sample_one | not available | sample_conditions_one |
| Flipback NOESY | sample_one | not available | sample_conditions_one |
| 15N-edited NOESY,TOCSY (3D) | sample_one | not available | sample_conditions_one |
| HNCA | sample_one | not available | sample_conditions_one |
| HN(CO)CA | sample_one | not available | sample_conditions_one |
| HCCH-TOCSY | sample_one | not available | sample_conditions_one |
| 13C-edited NOESY (3D) | sample_one | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
Related Database Links:
| BMRB | 1551 2278 4154 |
| PDB | |
| DBJ | BAA00414 BAN13288 |
| GB | AAA25759 |
| REF | WP_032492635 YP_009083114 |
| SP | P00259 |
| AlphaFold | P00259 |
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