BMRB Entry 4154
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4154
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Title: 13C and Stereospecific 13C and 1H Resonance Assignments for Oxidized Putidaredoxin
Deposition date: 1998-06-28 Original release date: 1999-09-28
Authors: Jain, Nitin; Lyons, Teresa; Pochapsky, Thomas; Kuti, Miklos
Citation: Pochapsky, Thomas; Jain, Nitin; Lyons, Teresa; Kuti, Miklos. "A Refined Model for the Solution Structure of Oxidized Putidaredoxin" Biochemistry 38, 4681-4690 (1999).
Assembly members:
putidaredoxin, polymer, 106 residues, Formula weight is not available
FES, non-polymer, 175.820 Da.
Natural source: Common Name: Pseudomonas putida Taxonomy ID: 303 Superkingdom: Eubacteria Kingdom: not available Genus/species: Pseudomonas putida
Experimental source: Production method: .
Entity Sequences (FASTA):
putidaredoxin: SKVVYVSHDGTRRELDVADG
VSLMQAAVSNGIYDIVGDCG
GSASCATCHVYVNEAFTDKV
PAANEREIGMLECVTAELKP
NSRLCCQIIMTPELDGIVVD
VPDRQW
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 376 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 625 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Pdxo | 1 |
| 2 | 2Fe2S | 2 |
Entities:
Entity 1, Pdxo 106 residues - Formula weight is not available
| 1 | SER | LYS | VAL | VAL | TYR | VAL | SER | HIS | ASP | GLY | ||||
| 2 | THR | ARG | ARG | GLU | LEU | ASP | VAL | ALA | ASP | GLY | ||||
| 3 | VAL | SER | LEU | MET | GLN | ALA | ALA | VAL | SER | ASN | ||||
| 4 | GLY | ILE | TYR | ASP | ILE | VAL | GLY | ASP | CYS | GLY | ||||
| 5 | GLY | SER | ALA | SER | CYS | ALA | THR | CYS | HIS | VAL | ||||
| 6 | TYR | VAL | ASN | GLU | ALA | PHE | THR | ASP | LYS | VAL | ||||
| 7 | PRO | ALA | ALA | ASN | GLU | ARG | GLU | ILE | GLY | MET | ||||
| 8 | LEU | GLU | CYS | VAL | THR | ALA | GLU | LEU | LYS | PRO | ||||
| 9 | ASN | SER | ARG | LEU | CYS | CYS | GLN | ILE | ILE | MET | ||||
| 10 | THR | PRO | GLU | LEU | ASP | GLY | ILE | VAL | VAL | ASP | ||||
| 11 | VAL | PRO | ASP | ARG | GLN | TRP |
Entity 2, 2Fe2S - Fe2 S2 - 175.820 Da.
| 1 | FES |
Samples:
sample_one: putidaredoxin, [U-13C, U-15N], 1 mM; deutero-trisbuffer
sample_conditions_one: pH: 7.4 na; temperature: 290 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | sample_one | not available | sample_conditions_one |
| HNCO | sample_one | not available | sample_conditions_one |
| HCCH-TOCSY | sample_one | not available | sample_conditions_one |
| 1H-13C HSQC | sample_one | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
Related Database Links:
| BMRB | 1551 2278 4149 |
| PDB | |
| DBJ | BAA00414 BAN13288 |
| GB | AAA25759 |
| REF | WP_032492635 YP_009083114 |
| SP | P00259 |
| AlphaFold | P00259 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts