BMRB Entry 50234

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50234

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Title:
15N-spin relaxation data for TRBP2-dsRBD1 dynamics in presence of D10-RNA
Deposition date:
2020-04-14
Original release date:
2022-02-16
Authors:
Paithankar, Harshad; Chugh, Jeetender
Citation:

Citation: Paithankar, Harshad; Tarang, Guneet Singh; Parvez, Firdousi; Marathe, Aniket; Joshi, Manali; Chugh, Jeetender. "Relay of intrinsic microsecond timescale conformational dynamics observed in an RNA-binding domain of TRBP upon enthalpy-driven binding of dsRNA"  Biophys. J. ., .-. (2022).
PubMed: 35134335

Assembly members:

Assembly members:
entity_1, polymer, 108 residues, Formula weight is not available
entity_2, polymer, 10 residues, Formula weight is not available
entity_3, polymer, 10 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSV272

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGAMSEEEQGSGTTTGCGLP SIEQMLAANPGKTPISLLQE YGTRIGKTPVYDLLKAEGQA HQPNFTFRVTVGDTSCTGQG PSKKAAKHKAAEVALKHLKG GSMLEPAL
entity_2: UUAUAAAUGC
entity_3: GCAUUUAUGA

Data sets:
Data typeCount
T1 relaxation values58
T2 relaxation values58
heteronuclear NOE values58

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1TRBP2-dsRBD11
2D10-RNA, chain 12
3D10-RNA, chain 23

Entities:

Entity 1, TRBP2-dsRBD1 108 residues - Formula weight is not available

N-terminal residues GGA are non-native to the protein and are left after the cleavage of the fusion tag using protease.

1   GLYGLYALAMETSERGLUGLUGLUGLNGLY
2   SERGLYTHRTHRTHRGLYCYSGLYLEUPRO
3   SERILEGLUGLNMETLEUALAALAASNPRO
4   GLYLYSTHRPROILESERLEULEUGLNGLU
5   TYRGLYTHRARGILEGLYLYSTHRPROVAL
6   TYRASPLEULEULYSALAGLUGLYGLNALA
7   HISGLNPROASNPHETHRPHEARGVALTHR
8   VALGLYASPTHRSERCYSTHRGLYGLNGLY
9   PROSERLYSLYSALAALALYSHISLYSALA
10   ALAGLUVALALALEULYSHISLEULYSGLY
11   GLYSERMETLEUGLUPROALALEU

Entity 2, D10-RNA, chain 1 10 residues - Formula weight is not available

5'-UUAUAAAUGC-3'

1   UUAUAAAUGC

Entity 3, D10-RNA, chain 2 10 residues - Formula weight is not available

5'-GCAUUUAUGA-3'

1   GCAUUUAUGA