BMRB Entry 50846
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50846
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Title: Imino resonance assignment of murine Ox40 mRNA 3'UTR PubMed: 35357505
Deposition date: 2021-03-24 Original release date: 2022-03-15
Authors: Tants, Jan-Niklas; Schlundt, Andreas
Citation: Tants, Jan-Niklas; Becker, Lea Marie; McNicoll, Francois; Muller-McNicoll, Michaela; Schlundt, Andreas. "NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin" Nucleic Acids Res. 50, 4083-4099 (2022).
Assembly members:
entity_1, polymer, 157 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: enzymatic semisynthesis
Entity Sequences (FASTA):
entity_1: GCAUUACUACAGGAGUGGAU
UUUAUGGGGCACGGACAACC
CAUAUCCUGAUGCCUGCCAG
UACCCUCCACACCGUUCUAG
GUGCUGGGCUGGCUCUGGGC
UUUCCUAUGUAUGCUAUGCA
UACUACCUGCCUGGUGGUGC
UCCUAAUAAACAUGCUA
- assigned_chemical_shifts
Data type | Count |
15N chemical shifts | 33 |
1H chemical shifts | 35 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Ox40 mRNA 3'UTR | 1 |
Entities:
Entity 1, Ox40 mRNA 3'UTR 157 residues - Formula weight is not available
1 | G | C | A | U | U | A | C | U | A | C | ||||
2 | A | G | G | A | G | U | G | G | A | U | ||||
3 | U | U | U | A | U | G | G | G | G | C | ||||
4 | A | C | G | G | A | C | A | A | C | C | ||||
5 | C | A | U | A | U | C | C | U | G | A | ||||
6 | U | G | C | C | U | G | C | C | A | G | ||||
7 | U | A | C | C | C | U | C | C | A | C | ||||
8 | A | C | C | G | U | U | C | U | A | G | ||||
9 | G | U | G | C | U | G | G | G | C | U | ||||
10 | G | G | C | U | C | U | G | G | G | C | ||||
11 | U | U | U | C | C | U | A | U | G | U | ||||
12 | A | U | G | C | U | A | U | G | C | A | ||||
13 | U | A | C | U | A | C | C | U | G | C | ||||
14 | C | U | G | G | U | G | G | U | G | C | ||||
15 | U | C | C | U | A | A | U | A | A | A | ||||
16 | C | A | U | G | C | U | A |
Samples:
sample_1: Ox40 3'UTR CDE 280 uM
sample_2: Ox40 3'UTR Terminus 440 uM
sample_3: Ox40 3'UTR Bulge 350 uM
sample_4: Ox40 3'UTR ADE 600 uM
sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 283 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1D 1H | sample_2 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
2D 1H-15N SOFAST HMQC | sample_2 | isotropic | sample_conditions_1 |
1D 1H | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N SOFAST HMQC | sample_1 | isotropic | sample_conditions_1 |
1D 1H | sample_3 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_3 | isotropic | sample_conditions_1 |
2D 1H-15N SOFAST HMQC | sample_3 | isotropic | sample_conditions_1 |
1D 1H | sample_4 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_4 | isotropic | sample_conditions_1 |
2D 1H-15N SOFAST HMQC | sample_4 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4.0.6 - processing
NMRFAM-SPARKY v1.470 - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker AVANCE III 800 MHz
- Bruker AVANCE III 600 MHz
- Bruker AVANCE III 700 MHz
- Bruker Avance II 600 MHz