BMRB Entry 6875

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6875

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Title:
Solution structure of a biologically active human FGF-1 monomer, complexed to a hexasaccharide heparin-analogue
Deposition date:
2005-10-25
Original release date:
2006-11-16
Authors:
Canales, Angeles; Lozano, Rosa; Nieto, Pedro; Martin-Lomas, Manuel; Gimenez-Gallego, Guillermo; Jimenez-Barbero, Jesus
Citation:

Citation: Canales, Angeles; Lozano, Rosa; Lopez-Mendez, B.; Angulo, J.; Ojeda, R.; Nieto, Pedro; Martin-Lomas, Manuel; Gimenez-Gallego, Guillermo; Jimenez-Barbero, Jesus. "Solution NMR structure of a human FGF-1 monomer, activated by a hexasaccharide heparin-analogue"  FEBS J. 273, 4716-4727 (2006).
PubMed: 16995857

Assembly members:

Assembly members:
FGF-1, polymer, 139 residues, 15755 Da.
HLHS, polymer, 7 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRAT4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FGF-1: NLPPGNYKKPKLLYCSNGGH FLRILPDGTVDGTRDRSDQH IQLQLSAESVGEVYIKSTET GQYLAMDTDGLLYGSQTPNE ECLFLERLEENHYNTYISKK HAEKNWFVGLKKNGSCKRGP RTHYGQKAILFLPLPVSSD
HLHS: XXXXXXX

Data sets:
Data typeCount
13C chemical shifts235
15N chemical shifts125
1H chemical shifts780

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FGF-11
2HLHS2

Entities:

Entity 1, FGF-1 139 residues - 15755 Da.

1   ASNLEUPROPROGLYASNTYRLYSLYSPRO
2   LYSLEULEUTYRCYSSERASNGLYGLYHIS
3   PHELEUARGILELEUPROASPGLYTHRVAL
4   ASPGLYTHRARGASPARGSERASPGLNHIS
5   ILEGLNLEUGLNLEUSERALAGLUSERVAL
6   GLYGLUVALTYRILELYSSERTHRGLUTHR
7   GLYGLNTYRLEUALAMETASPTHRASPGLY
8   LEULEUTYRGLYSERGLNTHRPROASNGLU
9   GLUCYSLEUPHELEUGLUARGLEUGLUGLU
10   ASNHISTYRASNTHRTYRILESERLYSLYS
11   HISALAGLULYSASNTRPPHEVALGLYLEU
12   LYSLYSASNGLYSERCYSLYSARGGLYPRO
13   ARGTHRHISTYRGLYGLNLYSALAILELEU
14   PHELEUPROLEUPROVALSERSERASP

Entity 2, HLHS 7 residues - Formula weight is not available

1   IDSGNSIDRNGYIDSGNSIPA

Samples:

sample-1: acidic fibroblast growth factor, [U-95% 15N], 1 mM; heparin like hexasaccharide 1 mM; sodium phosphate 10 mM; sodium chloride 150 mM; 3-trimethylsilylpropionate 1 mM

sample-2: acidic fibroblast growth factor, [U-95% 13C; U-95% 15N], 1 mM; heparin like hexasaccharide 1 mM; sodium phosphate 10 mM; sodium chloride 150 mM; 3-trimethylsilylpropionate 1 mM

conditions-1: ionic strength: 0.17 M; pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
NOESYnot availablenot availableconditions-1
1H-15N HSQCnot availablenot availableconditions-1
1H-15N NOESY-HSQCnot availablenot availableconditions-1
1H-15N TOCSY-HSQCnot availablenot availableconditions-1
HNCOnot availablenot availableconditions-1
HNCAnot availablenot availableconditions-1
HN(CO)CAnot availablenot availableconditions-1

Software:

XWINNMR v3.2 - data collection, processing

VNMR v1.1d - Data collection and processing

XEASY v1.3.13 - Data analysis

DYANA v1.5 - Structure calculation

AMBER v5.0 - Structure refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz
  • Varian Inova 750 MHz

Related Database Links:

BMRB 15783 15960 16493 16494 16502 17464 17674
PDB
DBJ BAF82451 BAG35227 BAI46827
EMBL CAA36206 CAA42869 CAA46661 CAI29610 CCE35537
GB AAA52446 AAA52638 AAA79245 AAB29057 AAH32697
PRF 1605206A
REF NP_000791 NP_001127073 NP_001138364 NP_001138406 NP_001138407
SP P05230 P20002 P34004 Q5NVQ3
AlphaFold P05230 P20002 P34004 Q5NVQ3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks