BMRB Entry 6878
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6878
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Title: Backbone 1H and 13C Chemical Shifts Assignemts for putative TM7 from V-ATPase subunit a PubMed: 16962559
Deposition date: 2005-10-25 Original release date: 2007-03-28
Authors: Duarte, Afonso; Wolfs, Cor; Nico, van Nuland; Michael, Harrison; John, Findlay; Carlo, van Mierlo; Marcus, Hemminga
Citation: Duarte, Afonso; Wolfs, Cor; Nico, van Nuland; Harrison, Michael; Findlay, John; van Mierlo, Carlo; Hemminga, Marcus. "Structure and localization of an essential transmembrane segment of the proton translocation channel of yeast H+-V-ATPase" Biochim. Biophys. Acta 1768, 218-227 (2007).
Assembly members:
MTM7, polymer, 37 residues, Formula weight is not available
Natural source: Common Name: yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
MTM7: IHTIEFCLNCVSHTASYLRL
WALSLAHAQLSSVLWTM
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 14 |
| 1H chemical shifts | 35 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MTM7 | 1 |
Entities:
Entity 1, MTM7 37 residues - Formula weight is not available
| 1 | ILE | HIS | THR | ILE | GLU | PHE | CYS | LEU | ASN | CYS | ||||
| 2 | VAL | SER | HIS | THR | ALA | SER | TYR | LEU | ARG | LEU | ||||
| 3 | TRP | ALA | LEU | SER | LEU | ALA | HIS | ALA | GLN | LEU | ||||
| 4 | SER | SER | VAL | LEU | TRP | THR | MET |
Samples:
MTM7: MTM7 2.0 nM; DMSO 100%
conditions_1: pH: .; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | MTM7 | not available | conditions_1 |
| 2D 1H-1H NOESY | MTM7 | not available | conditions_1 |
| 2D 1H-13C HSQC | MTM7 | not available | conditions_1 |
| 2D 1H-13C HMBC | MTM7 | not available | conditions_1 |
| TOCSY | MTM7 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 750 MHz
- Bruker AVANCE 600 MHz