BMRB Entry 7342
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7342
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Title: SOLUTION STRUCTURE OF THE ZN COMPLEX OF HIV-2 NCP(23-49) PEPTIDE, ENCOMPASSING PROTEIN CCHC-LINKER, DISTAL CCHC ZN-BINDING MOTIF AND C-TERMINAL TAIL
Deposition date: 2006-12-01 Original release date: 2011-05-26
Authors: Amodeo, P.; Castiglione Morelli, M.; Ostuni, A.; Cristinziano, P.; Bavoso, A.
Citation: Amodeo, P.; Castiglione Morelli, M.; Ostuni, A.; Cristinziano, P.; Bavoso, A.. "Structural Features of the C-Terminal Zinc Finger Domain of the HIV-2 Nc Protein (Residues 23-49)." .
Assembly members:
NUCLEOCAPSID_PROTEIN, polymer, 27 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: HIV-2 Taxonomy ID: 11709 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus HIV-2
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
NUCLEOCAPSID_PROTEIN: RAPRRQGCWKCGKTGHVMAK
CPERQAG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 184 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NUCLEOCAPSID PROTEIN | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, NUCLEOCAPSID PROTEIN 27 residues - Formula weight is not available
| 1 | ARG | ALA | PRO | ARG | ARG | GLN | GLY | CYS | TRP | LYS | ||||
| 2 | CYS | GLY | LYS | THR | GLY | HIS | VAL | MET | ALA | LYS | ||||
| 3 | CYS | PRO | GLU | ARG | GLN | ALA | GLY |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample: NCp8 2.6 mM; H2O 80%; D2O 20%; TCEP.HCl 11.5 mM; zinc chloride 3.1 mM
sample_conditions_1: ionic strength: 130 mM; pH: 7.0; pressure: 1.0 atm; temperature: 298.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample | isotropic | sample_conditions_1 |
| 2D TOCSY | sample | isotropic | sample_conditions_1 |
Software:
AMBER, PEARLMAN,CASE,CALDWELL,ROSS,CHEATHAM, FERGUSON,SEIBEL,SINGH,WEINER,KOLLMAN - refinement, structure solution
Molmol v2K.2 - data analysis
VNMR v6.1C - processing
NMR spectrometers:
- VARIAN UNITYINOVA 500 MHz